Overview

  • Product nameDAB Substrate Kit
    See all DAB substrate kit kits
  • Product overview

    This product is available in a two component form consisting of a liquid, refrigerator stable DAB Chromogen and DAB Substrate. Once the two components are combined, the reagent can be used for up to six hours, making it ideal for automated stainers.

  • Notes

    Contains DAB in buffer. Reagent is acidic and can cause burns if skin contact occurs.

    Contains Hydrogen Peroxide in DAB substrate buffer. Avoid contact with skin and eyes.

  • Tested applicationsSuitable for: IHC-Pmore details

Properties

  • Relevance3,3' Diaminobenzidine (DAB) is a widely used chromogen for immunohistochemical staining. In the presence of peroxidase enzyme, DAB produces a brown precipitate that is insoluble in alcohol.
  • Alternative names
    • 3 3' Diaminobenzidine

Applications

Our Abpromise guarantee covers the use of ab64238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

DAB Substrate Kit images

  • Human mantel cell lymphoma II stained with rabbit monoclonal [SP4] to Cyclin D1 (ab16663) using DAB substrate kit (ab64238).

Protocols

References for DAB Substrate Kit (ab64238)

This product has been referenced in:
  • Lean QY  et al. Orally Administered Enoxaparin Ameliorates Acute Colitis by Reducing Macrophage-Associated Inflammatory Responses. PLoS One 10:e0134259 (2015). IHC ; Mouse . Read more (PubMed: 26218284) »
  • Jujo T  et al. The role of matrix metalloproteinase in the intimal sarcoma-like cells derived from endarterectomized tissues from a chronic thromboembolic pulmonary hypertension patient. PLoS One 9:e87489 (2014). IHC-P . Read more (PubMed: 24489925) »

See all 9 Publications for this product

Product Wall

IHC-P Mouse pancreas

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Ki67 IHC staining on mouse pancreas, blocking with 10% BSA, 1 hr ab incubation RT
Username

Abcam user community

Verified customer

Submitted Aug 16 2016

Thank you for contacting us.
The kit will work with the rabbit and mouse primary antibodies.
You can try citrate buffer pH for a start and Tris/EDTA in case the staining does not work. Please check the following for protocol and troubleshooti...

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Thank you for contacting us.
Heat mediated antigen retrieval method with microwave or pressure cooker is recommended for this ab.
The suitable detection system depends on the substrate you want to use. e.g. with DAB or AEC, we have following ...

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ab1002 Anti-Rabies Virus antibody looks very promising as it has been established for the use in IHC-P and should detect a wide range of rabies strains. As a starting dilution the customer can tr...

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Thank you for contacting us.

Here is the list with the requested items. However, please be aware that this is not a technical inquiry as all item could be found by using the search application on our website under the "reagents" ca...

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Thank you for contacting us. You are correct, these are the same product, just different sizes. The appropriate size will depend on how many slides you anticipate needing to stain. If you prepare the substrate/chromogen mixture as suggested in the prot...

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Thank you for your reply.

I looked over the protocol that you sent and it looks to be OK with me. I had a colleague look at it aswell and they thought it was good too.

If there is anything else I can help you with, or if you have an...

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Thank you for your reply and for providing those details.

I have now emailed you a proforma regarding the purchase of ab46667 and ab64238.

As for detailed step by step protocols, we do not have any that would meet your requirements,...

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Thank you for calling Abcam earlier today.

To be able to send a quote for the products you requested, please could you send me your shipping and billing address.

Once I get this information, I can send that quote out.

Also...

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Thank you for your reply.

This antibody can be at -80C in aliquots for storage, and this may extend the life of the antibody. Either -20C or -80C will be fine.

Please let me know if you have any further questions, and I look forward...

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1-10 of 19 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"