Anti-Dab1 antibody (ab16674)

Overview

  • Product nameAnti-Dab1 antibody
    See all Dab1 primary antibodies
  • Description
    Goat polyclonal to Dab1
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Fusion protein, corresponding to amino acids 1-179 of Mouse Dab1.

  • Positive control
    • 293 cells transfected with Dab1–HA tag IP. Dab1 Knockout brain p14 lysate and Dab1 Wild Type brain p14 lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab16674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 67 kDa).
IP Use a concentration of 5 - 10 µg/ml.

Target

  • FunctionAdapter molecule functioning in neural development. May regulate SIAH1 activity.
  • Sequence similaritiesContains 1 PID domain.
  • DomainThe PID domain specifically binds to the Asn-Pro-Xaa-Tyr(P) motif found in many tyrosine-phosphorylated proteins.
  • Post-translational
    modifications
    Phosphorylated on Tyr-198 and Tyr-220 upon reelin induction in embryonic neurons (By similarity). Also phosphorylated on Ser-524 independently of reelin signaling.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dab 1 antibody
    • Dab reelin signal transducer 1 antibody
    • Dab, reelin signal transducer, homolog 1 (Drosophila) antibody
    • Dab1 antibody
    • DAB1_HUMAN antibody
    • Disabled homolog 1 antibody
    • Disabled homolog 1 Drosophila antibody
    • Scm antibody
    • Scr antibody
    • Scrambler antibody
    • Yot antibody
    • Yotari antibody
    see all

Anti-Dab1 antibody images



  • Predicted band size : 67 kDa


    ab16674 detecting mouse Dab1 in brain extracts by Western blot. Lane 1: ab16674 (N terminal). Lane 2: ab16675 (C terminal).

References for Anti-Dab1 antibody (ab16674)

ab16674 has not yet been referenced specifically in any publications.

Product Wall

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (THP-1)
Total protein in input 50 µg
Specification THP-1
Treatment 200 nM PMA for 24 hours. Cells in sample 2 were treated with 300 uM hydrogen peroxide for 20 mins before being lysed.
Immuno-precipitation step Protein A/G
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Verified customer

Submitted Aug 07 2012

Thank you for confirming those details.

As requested, I have generated the testing discount code for you as below:

Discount code: xxxxx

Expiration date: 5th November 2012

Please orderab111684 as normalandquote thi...

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Thank you for contacting us yesterday in regards to antibodies to be used in immunoprecipitation of Dab1 from mouse and human samples.

Unfortunately, as discussed over the phone, we do not currently have any antibodies in our catalogue which ...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Adult retina)
Specification Adult retina
Fixative Formaldehyde
Blocking step Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5%
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Abcam user community

Verified customer

Submitted Jun 14 2006

Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"