Anti-Dab1 antibody (ab16675)

Overview

  • Product name
  • Description
    Goat polyclonal to Dab1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recognizes Dab1 of 48 kDa, corresponding to amino acids 399-555 of mouse Dab1 fusion protein.

  • Positive control
    • WB: 293 cells transfected with Dab1–HA tag. IP: Mouse p14 brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab16675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 67 kDa).
IP Use a concentration of 5 µg/ml.

Target

  • Function
    Adapter molecule functioning in neural development. May regulate SIAH1 activity.
  • Sequence similarities
    Contains 1 PID domain.
  • Domain
    The PID domain specifically binds to the Asn-Pro-Xaa-Tyr(P) motif found in many tyrosine-phosphorylated proteins.
  • Post-translational
    modifications
    Phosphorylated on Tyr-198 and Tyr-220 upon reelin induction in embryonic neurons (By similarity). Also phosphorylated on Ser-524 independently of reelin signaling.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dab 1 antibody
    • Dab reelin signal transducer 1 antibody
    • Dab, reelin signal transducer, homolog 1 (Drosophila) antibody
    • Dab1 antibody
    • DAB1_HUMAN antibody
    • Disabled homolog 1 antibody
    • Disabled homolog 1 Drosophila antibody
    • Scm antibody
    • Scr antibody
    • Scrambler antibody
    • Yot antibody
    • Yotari antibody
    see all

Images



  • Predicted band size : 67 kDa

    ab16675 at 1µg/ml staining mouse Dab1 (80kDa) in mouse brain extract by Western blot.

    Lane 1: ab16674 (N terminal).

    Lane 2: ab16675 (C terminal).

    ab16675 at 1µg/ml staining mouse Dab1 (80kDa) in mouse brain extract by Western blot. Lane 1: ab16674 (N terminal). Lane 2: ab16675 (C terminal).

References

ab16675 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for confirming those details.

As requested, I have generated the testing discount code for you as below:

Discount code: xxxxx

Expiration date: 5th November 2012

Please orderab111684 as normalandquote thi...

Read More

Thank you for contacting us yesterday in regards to antibodies to be used in immunoprecipitation of Dab1 from mouse and human samples.

Unfortunately, as discussed over the phone, we do not currently have any antibodies in our catalogue which ...

Read More
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Neuroblastoma Cells)
Loading amount
400000 cells
Specification
Neuroblastoma Cells
Gel Running Conditions
Reduced Denaturing (Gel 10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Mar 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up