Anti-Dab1 antibody (ab16675)

Overview

  • Product nameAnti-Dab1 antibody
    See all Dab1 primary antibodies
  • Description
    Goat polyclonal to Dab1
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recognizes Dab1 of 48 kDa, corresponding to amino acids 399-555 of mouse Dab1 fusion protein.

  • Positive control
    • WB: 293 cells transfected with Dab1–HA tag IP: Dab1 Knockout brain p14 lysate and Dab1 Wild Type brain p14 lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab16675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 67 kDa).
IP Use a concentration of 5 µg/ml.

Target

  • FunctionAdapter molecule functioning in neural development. May regulate SIAH1 activity.
  • Sequence similaritiesContains 1 PID domain.
  • DomainThe PID domain specifically binds to the Asn-Pro-Xaa-Tyr(P) motif found in many tyrosine-phosphorylated proteins.
  • Post-translational
    modifications
    Phosphorylated on Tyr-198 and Tyr-220 upon reelin induction in embryonic neurons (By similarity). Also phosphorylated on Ser-524 independently of reelin signaling.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dab 1 antibody
    • Dab reelin signal transducer 1 antibody
    • Dab, reelin signal transducer, homolog 1 (Drosophila) antibody
    • Dab1 antibody
    • DAB1_HUMAN antibody
    • Disabled homolog 1 antibody
    • Disabled homolog 1 Drosophila antibody
    • Scm antibody
    • Scr antibody
    • Scrambler antibody
    • Yot antibody
    • Yotari antibody
    see all

Anti-Dab1 antibody images



  • Predicted band size : 67 kDa


    ab16675 at 1µg/ml staining mouse Dab1 (80kDa) in mouse brain extract by Western blot. Lane 1: ab16674 (N terminal). Lane 2: ab16675 (C terminal).

References for Anti-Dab1 antibody (ab16675)

ab16675 has not yet been referenced specifically in any publications.

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Neuroblastoma Cells)
Loading amount 400000 cells
Specification Neuroblastoma Cells
Gel Running Conditions Reduced Denaturing (Gel 10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Mar 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"