Overview

  • Product name
    Anti-Dab1 (phospho Y232) antibody
    See all Dab1 primary antibodies
  • Description
    Rabbit polyclonal to Dab1 (phospho Y232)
  • Specificity
    This antibody detects endogenous levels of Dab1 only when phosphorylated at tyrosine 232.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized phosphopeptide derived from human Dab1 around the phosphorylation site of tyrosine 232 (G-V-YP-D-V).

  • Positive control
    • Extracts from LOVO cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab78200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
ELISA 1/20000.

Target

  • Function
    Adapter molecule functioning in neural development. May regulate SIAH1 activity.
  • Sequence similarities
    Contains 1 PID domain.
  • Domain
    The PID domain specifically binds to the Asn-Pro-Xaa-Tyr(P) motif found in many tyrosine-phosphorylated proteins.
  • Post-translational
    modifications
    Phosphorylated on Tyr-198 and Tyr-220 upon reelin induction in embryonic neurons (By similarity). Also phosphorylated on Ser-524 independently of reelin signaling.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dab 1 antibody
    • Dab reelin signal transducer 1 antibody
    • Dab, reelin signal transducer, homolog 1 (Drosophila) antibody
    • Dab1 antibody
    • DAB1_HUMAN antibody
    • Disabled homolog 1 antibody
    • Disabled homolog 1 Drosophila antibody
    • Scm antibody
    • Scr antibody
    • Scrambler antibody
    • Yot antibody
    • Yotari antibody
    see all

Images

  • All lanes : Anti-Dab1 (phospho Y232) antibody (ab78200) at 1/500 dilution

    Lane 1 : Extracts from LOVO cells
    Lane 2 : Extracts from LOVO cells plus 5µg immunizing peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 67 kDa
    Observed band size : 67 kDa

References

This product has been referenced in:
  • Rogers JT  et al. Reelin supplementation enhances cognitive ability, synaptic plasticity, and dendritic spine density. Learn Mem 18:558-64 (2011). Read more (PubMed: 21852430) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab78200.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up