Recombinant
RabMAb

Anti-Dab1 (phospho Y232) antibody [EPR2247(2)] (ab126728)

Overview

  • Product name
    Anti-Dab1 (phospho Y232) antibody [EPR2247(2)]
    See all Dab1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2247(2)] to Dab1 (phospho Y232)
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Phospho specific peptide corresponding to residues surrounding Tyrosine 232 of Human Dab1.

  • Positive control
    • SH-SY5Y cells and lysates treated with pervanadate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab126728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 80 kDa (predicted molecular weight: 64 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      Adapter molecule functioning in neural development. May regulate SIAH1 activity.
    • Sequence similarities
      Contains 1 PID domain.
    • Domain
      The PID domain specifically binds to the Asn-Pro-Xaa-Tyr(P) motif found in many tyrosine-phosphorylated proteins.
    • Post-translational
      modifications
      Phosphorylated on Tyr-198 and Tyr-220 upon reelin induction in embryonic neurons (By similarity). Also phosphorylated on Ser-524 independently of reelin signaling.
    • Information by UniProt
    • Database links
    • Alternative names
      • Dab 1 antibody
      • Dab reelin signal transducer 1 antibody
      • Dab, reelin signal transducer, homolog 1 (Drosophila) antibody
      • Dab1 antibody
      • DAB1_HUMAN antibody
      • Disabled homolog 1 antibody
      • Disabled homolog 1 Drosophila antibody
      • Scm antibody
      • Scr antibody
      • Scrambler antibody
      • Yot antibody
      • Yotari antibody
      see all

    Images

    • All lanes : Anti-Dab1 (phospho Y232) antibody [EPR2247(2)] (ab126728) at 1/10000 dilution

      Lane 1 : untreated SH-SY5Y cell lysate
      Lane 2 : pervanadate treated SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 64 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    • ab126728, at a dilution of 1/10 staining Dab1 (phospho Y232) in permeabilized pervanadate-treated (red) or untreated (negative) (green) SH-SY5Y cells by Flow Cytometry. Using ab126728 preincubated with phospho-Dab1 Phospho (pY232) peptide (blue) or non-phospho-Dab1 Phospho (pY232) peptide (orange).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Brai E  et al. Notch1 Regulates Hippocampal Plasticity Through Interaction with the Reelin Pathway, Glutamatergic Transmission and CREB Signaling. Front Cell Neurosci 9:447 (2015). WB ; Mouse . Read more (PubMed: 26635527) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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