Overview

  • Product name
    Anti-DAB2 antibody - C-terminal
    See all DAB2 primary antibodies
  • Description
    Rabbit polyclonal to DAB2 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human DAB2 aa 741-770 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P98082

  • Positive control
    • A2058 cell lysate, Human prostate carcinoma tissue, HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab175950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 82 kDa.

Target

  • Relevance
    DAB2 is a component of the CSF1 signal transduction pathway. DAB2 mRNA is expressed in normal ovarian epithelial cells but is down regulated or absent from ovarian carcinoma cell lines. The down regulation of DAB2 may play an important role in ovarian carcinogenesis. This gene was initially named DOC2 (for Differentially expressed in Ovarian Cancer) and is distinct from the DOC2A and DOC2B genes (for double C2 like domains, alpha and beta).
  • Cellular localization
    Cytoplasmic vesicle; clathrin coated vesicle; clathrin coated vesicle membrane. Cell membrane; coated pit; clathrin coated pit. Note: Colocalizes with large insert containing isoforms of MYO6 at clathrin coated pits/vesicles.
  • Database links
  • Alternative names
    • DAB 2 antibody
    • Dab, mitogen-responsive phosphoprotein, homolog 2 (Drosophila) antibody
    • DAB2 antibody
    • Differentially expressed in ovarian cancer 2 antibody
    • Differentially expressed protein 2 antibody
    • Disabled (Drosophila) homolog 2 (mitogen responsive phosphoprotein) antibody
    • Disabled homolog 2 antibody
    • Disabled homolog 2 mitogen responsive phosphoprotein (Drosophila) antibody
    • Disabled homolog 2 mitogen responsive phosphoprotein antibody
    • Disabled, Drosophila, homolog of, 2 antibody
    • DOC 2 antibody
    • DOC2 antibody
    • FLJ26626 antibody
    • Mitogen responsive phosphoprotein antibody
    see all

Images

  • Anti-DAB2 antibody - C-terminal (ab175950) at 1/1000 dilution + A2058 cell line lysate at 35 µg

    Predicted band size: 82 kDa

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded Human prostate carcinoma tissue, labeling DAB2 using ab175950 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometry analysis of HeLa cells labeling DAB2 (green, bottom histogram), using ab175950 at a 1/10 dilution, and negative control cells (blue, top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab175950 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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