Synthetic peptide within Human DAB2 aa 741-770 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: P98082
Our Abpromise guarantee covers the use of ab175950 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000. Predicted molecular weight: 82 kDa.|
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human prostate carcinoma tissue, labeling DAB2 using ab175950 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometry analysis of HeLa cells labeling DAB2 (green, bottom histogram), using ab175950 at a 1/10 dilution, and negative control cells (blue, top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ab175950 has not yet been referenced specifically in any publications.