Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
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Anti-DAB2 antibody [EP2297Y] (ab76253) at 1/2000 dilution + human pancreas tissue lysates at 10 µg
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 83 kDa
Observed band size : 105 kDa (why is the actual band size different from the predicted?)
Overlay histogram showing HeLa cells stained with ab76253 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76253, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (
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