Overview

  • Product name
  • Description
    Goat polyclonal to DACH1
  • Specificity
    ab106838 is expected to recognise all three reported isoforms(NP_542937.1; NP_542938.1; NP_004383.2).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to internal sequence amino acids 179-191 (

    SPVENTPQNNECK

    ) of Human DACH1 (NP_542937.1; NP_542938.1; NP_004383.2)

  • Positive control
    • Human kidney tissue lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 79 kDa.

Target

  • Function
    Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex. Transcriptional activation seems also to involve association of CREBBP. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter (By similarity). Inhibits TGF-beta signaling through interaction with SMAD4 and NCOR1. Binds to chromatin DNA via its DACHbox-N domain.
  • Tissue specificity
    Widely expressed. Isoform 2 is found in brain, heart, kidney, liver, leukocytes and spleen. Isoform 3 is found in liver and heart. Isoform 4 is found in spleen.
  • Sequence similarities
    Belongs to the DACH/dachshund family.
  • Domain
    The DACHbox-N/DD1 domain forms a structure containing a DNA binding motif similar to that of the forkhead/winged helix domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DACH antibody
    • Dach1 antibody
    • DACH1_HUMAN antibody
    • Dachshund family transcription factor 1 antibody
    • dachshund homolog 1 (Drosophila) antibody
    • Dachshund homolog 1 antibody
    • Dachsund homolog 1 antibody
    • FLJ10138 antibody
    • OTTHUMP00000018494 antibody
    see all

Images

  • Anti-DACH1 antibody (ab106838) at 0.1 µg/ml + Human Kidney lysate in RIPA buffer at 35 µg

    Predicted band size : 79 kDa
    Additional bands at : 52 kDa (possible isoform),57 kDa (possible isoform),73 kDa (possible isoform).

References

ab106838 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab106838.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up