• Product nameAnti-DACH1 antibody [EPR2841Y]
    See all DACH1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2841Y] to DACH1
  • Tested applicationsSuitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the C terminus of human DACH1

  • Positive control
    • MCF-7 and HeLa cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab77234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFlow Cyt: 1/120.
    IP: 1/40.
    WB: 1/500 - 1/1000.Predicted molecular weight: 79 kDa.

    Is unsuitable for ICC/IF or IHC-P.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex. Transcriptional activation seems also to involve association of CREBBP. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter (By similarity). Inhibits TGF-beta signaling through interaction with SMAD4 and NCOR1. Binds to chromatin DNA via its DACHbox-N domain.
    • Tissue specificityWidely expressed. Isoform 2 is found in brain, heart, kidney, liver, leukocytes and spleen. Isoform 3 is found in liver and heart. Isoform 4 is found in spleen.
    • Sequence similaritiesBelongs to the DACH/dachshund family.
    • DomainThe DACHbox-N/DD1 domain forms a structure containing a DNA binding motif similar to that of the forkhead/winged helix domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • DACH antibody
      • Dach1 antibody
      • DACH1_HUMAN antibody
      • dachshund homolog 1 (Drosophila) antibody
      • Dachshund homolog 1 antibody
      • Dachsund homolog 1 antibody
      • FLJ10138 antibody
      • OTTHUMP00000018494 antibody
      see all

    Anti-DACH1 antibody [EPR2841Y] images

    • All lanes : Anti-DACH1 antibody [EPR2841Y] (ab77234) at 1/1000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : Hela cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 79 kDa
      Observed band size : 97 kDa (why is the actual band size different from the predicted?)
    • Overlay histogram showing HeLa cells stained with ab77234 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77234, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    References for Anti-DACH1 antibody [EPR2841Y] (ab77234)

    This product has been referenced in:
    • Lutay N  et al. Bacterial control of host gene expression through RNA polymerase II. J Clin Invest 123:2366-79 (2013). WB ; Human . Read more (PubMed: 23728172) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Antigen Unmasking Solution - Vector h3300
    Sample Human Tissue sections (adrenal tumour)
    Specification adrenal tumour
    Permeabilization No
    Fixative Methanol

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    Submitted Sep 11 2013