Overview

  • Product nameAnti-DACH2 antibody
    See all DACH2 primary antibodies
  • Description
    Goat polyclonal to DACH2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Dog
  • Immunogen

    Synthetic peptide:

    TRKQAVNSSRPGR

    by a Cysteine residue linker, corresponding to internal sequence amino acids 169-181 of Human DACH2

  • Positive control
    • NIH3T3 cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab77688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesPeptide ELISA: Antibody detection limit dilution 1/64000.
    WB: Use at a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa according to mouse NP_291083.1 and 65 kDa according to human NP_444511.1).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscription factor that is involved in regulation of organogenesis. Seems to be a regulator for SIX1 and SIX6. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Is recruited with SIX6 to the p27Kip1 promoter in embryonal retina. SIX6 corepression seems also to involve NCOR1, TBL1, HDAC1 and HDAC3. May be involved together with PAX3, SIX1, and EYA2 in regulation of myogenesis. In the developing somite, expression of DACH2 and PAX3 is regulated by the overlying ectoderm, and DACH2 and PAX3 positively regulate each other's expression (By similarity). Probably binds to DNA via its DACHbox-N domain.
    • Sequence similaritiesBelongs to the DACH/dachshund family.
    • DomainThe DACHbox-N/DD1 domain forms a structure containing a DNA binding motif similar to that of the forkhead/winged helix domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • DACH 2 antibody
      • Dach2 antibody
      • DACH2_HUMAN antibody
      • Dachshund 2 antibody
      • Dachshund homolog 2 (Drosophila) antibody
      • Dachshund homolog 2 antibody
      • Dachshund2 antibody
      • FLJ31391 antibody
      • HGNC:16814 antibody
      • MGC138545 antibody
      see all

    Anti-DACH2 antibody images

    • Anti-DACH2 antibody (ab77688) at 0.03 µg/ml + NIH3T3 cell lysate (in RIPA buffer) at 35 µg
      Developed using the ECL technique

      Predicted band size : 69 kDa
      Observed band size : 69 kDa

    References for Anti-DACH2 antibody (ab77688)

    ab77688 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (Ovary)
    Antigen retrieval step Enzymatic
    Permeabilization No
    Specification Ovary
    Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: RT°C
    Fixative Paraformaldehyde
    Username

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    Verified customer

    Submitted Apr 14 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"