Anti-Dact1 / Dapper homolog 1 antibody (ab42547)

Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab42547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90,98 kDa (predicted molecular weight: 90 kDa).

Target

  • FunctionPositively regulates DVL2-mediated signaling pathways during development. Binds to DVL2 and impedes the degradation of CTNNB1/beta-catenin, thereby enhancing the transcriptional activation of target genes of the Wnt signaling pathway.
  • Sequence similaritiesBelongs to the dapper family.
  • DomainThe C-terminal PDZ-binding motif mediates interaction with the PDZ domains of DSH (Dishevelled) family proteins.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dact antibody
    • dact1 antibody
    • DACT1_HUMAN antibody
    • Dapper antagonist of beta catenin homolog 1 antibody
    • Dapper antagonist of catenin 1 antibody
    • Dapper antibody
    • Dapper homolog 1 antibody
    • DAPPER1 antibody
    • Dpr antibody
    • Dpr1 antibody
    • Frd antibody
    • Frodo antibody
    • hDPR1 antibody
    • Hepatocellular carcinoma novel gene 3 protein antibody
    • Heptacellular carcinoma novel gene 3 antibody
    • HNG3 antibody
    • MTNG3 antibody
    • Thyex3 antibody
    see all

Anti-Dact1 / Dapper homolog 1 antibody images

  • All lanes : Anti-Dact1 / Dapper homolog 1 antibody (ab42547) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 90 kDa
    Observed band size : 90,98 kDa (why is the actual band size different from the predicted?)
  • IHC image of Dact1/Dapper homolog 1 staining in human hippocampus FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab42547, 5µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • ICC/IF image of ab42547 stained A431 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42547, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Dact1 / Dapper homolog 1 antibody (ab42547)

ab42547 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Bone marrow and lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization Yes - De-parrafinization with xylene
Specification Bone marrow and lung
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Paraformaldehyde
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Submitted Nov 22 2016

Application Western blot
Sample Human Cell lysate - whole cell (Breast cancer)
Gel Running Conditions Reduced Denaturing
Loading amount 100000 cells
Specification Breast cancer
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Nov 22 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"