Anti-Dact1 / Dapper homolog 1 antibody (ab72078)

Overview

  • Product nameAnti-Dact1 / Dapper homolog 1 antibody
    See all Dact1 / Dapper homolog 1 primary antibodies
  • Description
    Rabbit polyclonal to Dact1 / Dapper homolog 1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Xenopus laevis, Chimpanzee, Zebrafish
  • Immunogen

    A portion of amino acids 725-775 of human Dact1/ Dapper homolog 1, (NP_057735).

  • Positive control
    • Human, mouse and rat brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab72078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesWB: Use at a concentration of 0.5 - 1 µg/ml. Detects bands of approximately 24 and 110 kDa (predicted molecular weight: 90 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-Dact1 / Dapper homolog 1 antibody images

    • ab72078 (10µg/ml) staining Dact1 / Dapper homolog 1 in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation in 10mM sodium citrate buffer pH6.0.

    • Lanes 1 - 2 : Anti-Dact1 / Dapper homolog 1 antibody (ab72078) at 1 µg/ml
      Lanes 3 - 4 : Anti-Dact1 / Dapper homolog 1 antibody (ab72078) at 0.5 µg/ml

      Lane 1 : Pre-immune sera
      Lane 2 : Human brain lysate
      Lane 3 : Mouse brain lysate
      Lane 4 : Rat brain lysate


      Predicted band size : 90 kDa
      Observed band size : 110 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 24 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-Dact1 / Dapper homolog 1 antibody (ab72078)

    ab72078 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guar...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"