This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Overview

  • Product name
  • Description
    Rabbit polyclonal to Dact2

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human, Xenopus laevis
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Mouse Dact2.

    (Peptide available as ab42375.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: Pancreas (Mouse) Tissue; NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell; Thymus (Mouse) Tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

  • Application notes
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 82 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Negatively regulates the Nodal signaling pathway, possibly by promoting the lysosomal degradation of Nodal receptors.
    • Sequence similarities
      Belongs to the dapper family.
    • Domain
      The C-terminal PDZ-binding motif may mediate interaction with the PDZ domains of DSH (Dishevelled) family proteins.
    • Information by UniProt
    • Database links
    • Alternative names
      • bA503C24.7 antibody
      • C6orf116 antibody
      • Chromosome 6 open reading frame 116 antibody
      • Dact2 antibody
      • DACT2_HUMAN antibody
      • Dapper antagonist of beta catenin homolog 2 antibody
      • Dapper antagonist of beta catenin homolog 2 Xenopus laevis antibody
      • Dapper homolog 2 antagonist of beta catenin antibody
      • Dapper homolog 2 antagonist of beta catenin xenopus antibody
      • Dapper homolog 2 antibody
      • DAPPER2 antibody
      • DPR2 antibody
      • FLJ31232 antibody
      • MGC133141 antibody
      • MGC133142 antibody
      • RP11 503C24.7 antibody
      see all

    Images

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • IHC image of Dact2 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab42099, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    • All lanes : Anti-Dact2 antibody (ab42099) at 1 µg/ml

      Lane 1 : Mouse pancreas tissue lysate - total protein (ab29363)
      Lane 2 : NIH 3T3 whole cell lysate (ab7179)
      Lane 3 : Mouse thymus tissue lysate - total protein (ab29285)

      Lysates/proteins at 10 µg per lane.

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 82 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-Dact2 antibody (ab42099) at 1 µg/ml

      Lane 1 : Kidney (Mouse) Tissue Lysate
      Lane 2 : Brain (Mouse) Tissue Lysate
      Lane 3 : Human kidney tissue lysate - total protein (ab30203)
      Lane 4 : Human brain tissue lysate - total protein (ab29466)
      Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 6 : SK N SH (Human neuroblastoma) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 82 kDa
      Observed band size : 115 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 2 minutes

    References

    ab42099 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab42099.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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