This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Overview

  • Product nameAnti-Dact2 antibody
    See all Dact2 primary antibodies
  • Description
    Rabbit polyclonal to Dact2

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Xenopus laevis
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Mouse Dact2.

    (Peptide available as ab42376.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: Pancreas (Mouse) Tissue; NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell; This antibody also gave a positive signal in Thymus (Mouse) Tissue - data not shown

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

  • Application notesWB: 1/250. Detects a band of approximately 115 kDa (predicted molecular weight: 82 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionNegatively regulates the Nodal signaling pathway, possibly by promoting the lysosomal degradation of Nodal receptors.
    • Sequence similaritiesBelongs to the dapper family.
    • DomainThe C-terminal PDZ-binding motif may mediate interaction with the PDZ domains of DSH (Dishevelled) family proteins.
    • Information by UniProt
    • Database links
    • Alternative names
      • bA503C24.7 antibody
      • C6orf116 antibody
      • Chromosome 6 open reading frame 116 antibody
      • Dact2 antibody
      • DACT2_HUMAN antibody
      • Dapper antagonist of beta catenin homolog 2 antibody
      • Dapper antagonist of beta catenin homolog 2 Xenopus laevis antibody
      • Dapper homolog 2 antagonist of beta catenin antibody
      • Dapper homolog 2 antagonist of beta catenin xenopus antibody
      • Dapper homolog 2 antibody
      • DAPPER2 antibody
      • DPR2 antibody
      • FLJ31232 antibody
      • MGC133141 antibody
      • MGC133142 antibody
      • RP11 503C24.7 antibody
      see all

    Anti-Dact2 antibody images

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • All lanes : Anti-Dact2 antibody (ab42377) at 1 µg/ml

      Lane 1 : Kidney (Mouse) Tissue Lysate
      Lane 2 : Brain (Mouse) Tissue Lysate
      Lane 3 : Human kidney tissue lysate - total protein (ab30203)
      Lane 4 : Human brain tissue lysate - total protein (ab29466)
      Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 6 : SK N SH (Human neuroblastoma) Whole Cell

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Observed band size : 115 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 1 minute
    • All lanes : Anti-Dact2 antibody (ab42377) at 1/250 dilution

      Lane 1 : Mouse pancreas tissue lysate - total protein (ab29363)
      Lane 2 : NIH 3T3 whole cell lysate (ab7179)

      Lysates/proteins at 10 µg per lane.

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Observed band size : 115 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 37 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-Dact2 antibody (ab42377)

    ab42377 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab42377.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"