The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 12 kDa.
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at an assay dependent concentration.
FunctionComponent of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis.
Sequence similaritiesBelongs to the DAD/OST2 family.
ab116226, at 2.5 µg/ml, staining DAD1 in formalin fixed, paraffin embedded Human prostate tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with biotinylated goat anti-rabbit IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
ab116226, at 2.5 µg/ml, staining DAD1 in formalin fixed, paraffin embedded Human brain (cerebellum) tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with biotinylated goat anti-rabbit IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
References for Anti-DAD1 antibody (ab116226)
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