Overview

  • Product name
  • Description
    Rabbit polyclonal to DAD1
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Syntheic peptide corresponding to 14 amino acids near the C terminus of Human DAD1.

  • Positive control
    • IHC-P: Human prostate and brain (cerebellum) tissues

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -80°C.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Ion Exchange Chromatography
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab116226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 12 kDa.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration.

Target

  • Function
    Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis.
  • Sequence similarities
    Belongs to the DAD/OST2 family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DAD 1 antibody
    • DAD-1 antibody
    • dad1 antibody
    • DAD1_HUMAN antibody
    • Defender against cell death 1 antibody
    • Dolichyl diphosphooligosaccharide protein glycosyltransferase subunit DAD1 antibody
    • Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 antibody
    • Oligosaccharyl transferase subunit DAD1 antibody
    • OST 2 antibody
    • OST2 antibody
    see all

Images

  • ab116226, at 2.5 µg/ml, staining DAD1 in formalin fixed, paraffin embedded Human prostate tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with biotinylated goat anti-rabbit IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • ab116226, at 2.5 µg/ml, staining DAD1 in formalin fixed, paraffin embedded Human brain (cerebellum) tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with biotinylated goat anti-rabbit IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

References

ab116226 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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