The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 74 kDa.Can be blocked with Human DAGLB peptide (ab104607). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionCatalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in tissues. Required for axonal growth during development and for retrograde synaptic signaling at mature synapses.
Sequence similaritiesBelongs to the AB hydrolase superfamily. Lipase family.