Overview

  • Product nameAnti-DAK antibody
    See all DAK primary antibodies
  • Description
    Rabbit polyclonal to DAK
  • SpecificityThis antibody detects endogenous levels of total DAK protein.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthesized peptide derived from the internal region of human DAK.

  • Positive control
    • Extracts from K562 cells and A549 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab66006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: 1/20000.
    WB: 1/500 - 1/1000. Detects bands of approximately 59 and 26 kDa (predicted molecular weight: 59 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCatalyzes both the phosphorylation of dihydroxyacetone and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate.
    • Sequence similaritiesBelongs to the dihydroxyacetone kinase (DAK) family.
      Contains 1 DhaK domain.
      Contains 1 DhaL domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATP dependent dihydroxyacetone kinase antibody
      • Bifunctional ATP dependent dihydroxyacetone kinase/FAD AMP lyase (cyclizing) antibody
      • DAK antibody
      • DHA kinase antibody
      • Dha kinase/FMN cyclase antibody
      • DHAK_HUMAN antibody
      • Dihydroxyacetone kinase antibody
      • Dihydroxyacetone kinase 2 antibody
      • Dihydroxyacetone kinase 2 homolog (S. cerevisiae) antibody
      • Dihydroxyacetone kinase 2 homolog (yeast) antibody
      • FAD AMP lyase (cyclic FMN forming) antibody
      • FAD AMP lyase (cyclizing) antibody
      • FAD-AMP lyase (cyclic FMN forming) antibody
      • FAD-AMP lyase (cyclizing) antibody
      • FMN cyclase antibody
      • Glycerone kinase antibody
      see all

    Anti-DAK antibody images

    • All lanes : Anti-DAK antibody (ab66006) at 1/500 dilution

      Lane 1 : Extracts from K562 cells
      Lane 2 : Extracts from A549 cells
      Lane 3 : Extracts from A549 cells, plus 5µg immunizing peptide

      Lysates/proteins at 5 µg per lane.


      Predicted band size : 59 kDa
      Observed band size : 59 kDa
      Additional bands at : 26 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-DAK antibody (ab66006)

    ab66006 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab66006.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"