Overview

  • Product nameAnti-Dally antibody
  • Description
    Mouse polyclonal to Dally
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Drosophila melanogaster
  • Immunogen

    Fusion protein:

    DLDGIHHHQHHLHSATTHHRRRLQRDSRAKDAVGG

    , corresponding to amino acids 30/64 of Drosophila melanogaster Dally

  • General notes

    Produced from outbred CD1 mice

     

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferConstituents: 50% Glycerol
  • PurityWhole antiserum
  • Primary antibody notesThis antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21806980
WB 1/1000. Predicted molecular weight: 69 kDa.

Target

  • FunctionCell surface proteoglycan that bears heparan sulfate. Required for cell division patterning during post-embryonic development of the nervous system.
  • Sequence similaritiesBelongs to the glypican family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • CG4974 antibody
      • dally antibody
      • Dally protein antibody
      • DALY_DROME antibody
      • Division abnormally delayed protein antibody
      see all

    Anti-Dally antibody images

    • All lanes : Anti-Dally antibody (ab22008) at 1/1000 dilution

      Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
      Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

      Secondary
      Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

      Predicted band size : 69 kDa

    References for Anti-Dally antibody (ab22008)

    This product has been referenced in:
    • Wojcinski A  et al. DSulfatase-1 fine-tunes Hedgehog patterning activity through a novel regulatory feedback loop. Dev Biol 358:168-80 (2011). ICC/IF ; Drosophila melanogaster . Read more (PubMed: 21806980) »
    • Nauck MA  et al. Dapagliflozin versus glipizide as add-on therapy in patients with type 2 diabetes who have inadequate glycemic control with metformin: a randomized, 52-week, double-blind, active-controlled noninferiority trial. Diabetes Care 34:2015-22 (2011). IHC (PFA fixed) ; Drosophila melanogaster . Read more (PubMed: 21816980) »

    See all 3 Publications for this product

    Product Wall

    Unfortunately, it is not possible that we receive this antibody in a purified and glycerol free form, therefore, we cannot conjugate the antibody.

    I am sorry to disappoint you.

    Thank you for contacting us.

    Unfortunately, it is impossible to conjugate this antibody for two reasons:

    1. This antibody contains 50% Glycerol, and it is impossible to conjugate an antibody in the presence of Glycerol. This would ...

    Read More
    Application Western blot
    Sample Fruit fly (Drosophila melanogaster) Cell lysate - other (S2 cells)
    Loading amount 15 µg
    Specification S2 cells
    Gel Running Conditions Reduced Denaturing (8% gel)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
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    Verified customer

    Submitted Sep 25 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"