Anti-DAP Kinase 1 (phospho S308) antibody [DKPS308] (ab10524)

Overview

  • Product nameAnti-DAP Kinase 1 (phospho S308) antibody [DKPS308]
    See all DAP Kinase 1 primary antibodies
  • Description
    Mouse monoclonal [DKPS308] to DAP Kinase 1 (phospho S308)
  • Tested applicationsSuitable for: ELISA, IP, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 303-312 (pSER308) of Human DAP Kinase 1.

Properties

Applications

Our Abpromise guarantee covers the use of ab10524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
IP
Dot blot
  • Application notesDot: Use at a concentration of 1 - 2 µg/ml, Determined by immunoblotting using 293T (human embryonal kidney) cells transfected with DAPKinase expression vector.
    ELISA: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCalcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
      Contains 10 ANK repeats.
      Contains 1 death domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Ubiquitinated by the BCR(KLHL20) E3 ubiquitin ligase complex, leading to its degradation by the proteasome.
    • Cellular localizationCytoplasm. Colocalizes with the actin filament system.
    • Information by UniProt
    • Database links
    • Alternative names
      • DAK1 antibody
      • DAP K1 antibody
      • DAP kinase 1 antibody
      • DAPK 1 antibody
      • DAPK antibody
      • DAPK1 antibody
      • DAPK1_HUMAN antibody
      • Death Associated Protein Kinase 1 antibody
      • Death-associated protein kinase 1 antibody
      • DKFZp781I035 antibody
      see all

    References for Anti-DAP Kinase 1 (phospho S308) antibody [DKPS308] (ab10524)

    ab10524 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - nuclear (EAhy926 endothelial cell line, HUVECs, HEK293, HeL)
    Gel Running Conditions Reduced Denaturing (12%)
    Loading amount 20 µg
    Treatment Alkaline Phosphatase treated, TNF-alpha treated, Vanadate treated, NaF treated, H2O2 treated, Ceramide treated
    Specification EAhy926 endothelial cell line, HUVECs, HEK293, HeL
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
    Username

    Dr. Nima Abbasian

    Verified customer

    Submitted Jan 28 2016

    Thank you for your reply.
    Unfortunately we can not send out free trial vials. We do also not have small sample sizes of our products.
    I am very sorry for this.
    I would like to remind you, that in the testing offer explained to you in my ...

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    Thank you very much for your interest in ab10524.
    To our knowledge, ab10524 has not been tested in ICC/IF. Therefore, I can offer a discount off a future purchase if you buy ab10524 now, test it in ICC/IF and submit feedback to us in the form of a...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"