Overview

  • Product name
    Anti-DAP Kinase 2 antibody
    See all DAP Kinase 2 primary antibodies
  • Description
    Rabbit polyclonal to DAP Kinase 2
  • Specificity
    Does not react with DAPK1.
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Peptide (

    ARRKALHPRRRSSTS

    ) corresponding to aminoacids 356 to 370 of human DAPK2 (1,2). The sequence ofthis antigenic peptide is identical to the correspondingamino acids of mouse origin (1,2)

  • Positive control
    • A431 whole cell lysate. An approximately42 kDa band can be detected.
  • General notes


    Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as RIP and DAP kinase, are mediators of apoptosis. DAP kinase (DAPK) is pro-apoptotic calcium-regulated serine/threonine kinase containing death domain. Ectopic expression of DAPK induces cell death and suppresses oncogenic transformation. DAPK mediates IFNg induced apoptosis. A novel DAP kinase-related protein was recently identified and designated DAPK2 and DRP-1 (1, 2). Ectopicly expressed DAPK2 induced apoptosis in various types ofcells (1,2). DAPK has high sequence homology to ZIP kinase and DRAK1/2, and they represent a novel family ofserine/threonine kinases, which mediates apoptosis through their catalytic activities. The messenger RNA of DAPK2 is expressed in multiple human tissues (1).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    PBS with 0.02% sodium azide
  • Concentration information loading...
  • Purity
    IgG fraction
  • Primary antibody notes
    Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as RIP and DAP kinase, are mediators of apoptosis. DAP kinase (DAPK) is pro-apoptotic calcium-regulated serine/threonine kinase containing death domain. Ectopic expression of DAPK induces cell death and suppresses oncogenic transformation. DAPK mediates IFNg induced apoptosis. A novel DAP kinase-related protein was recently identified and designated DAPK2 and DRP-1 (1, 2). Ectopicly expressed DAPK2 induced apoptosis in various types ofcells (1,2). DAPK has high sequence homology to ZIP kinase and DRAK1/2, and they represent a novel family ofserine/threonine kinases, which mediates apoptosis through their catalytic activities. The messenger RNA of DAPK2 is expressed in multiple human tissues (1).
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 µg/ml.
WB Use at an assay dependent concentration. Can be blocked with DAP Kinase 2 peptide (ab8436).

Target

  • Function
    Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.
  • Tissue specificity
    Ubiquitously expressed in all tissue types examined. High levels in heart, lung and skeletal muscle.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • DAK2 antibody
    • DAP kinase 2 antibody
    • DAP kinase related protein 1 antibody
    • DAP kinase related protein 1 beta isoform antibody
    • DAP-kinase-related protein 1 antibody
    • DAPK2 antibody
    • DAPK2_HUMAN antibody
    • Death associated protein kinase 2 antibody
    • Death-associated protein kinase 2 antibody
    • DRP 1 antibody
    • DRP-1 antibody
    • DRP1 antibody
    • MGC119312 antibody
    see all

Images

  • All lanes : Anti-DAP Kinase 2 antibody (ab8404) at 1 µg/ml

    Lane 1 : A431 whole cell lysate
    Lane 2 : Mouse spleen lysate
    Lane 3 : Rat kidney lysate


    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • ab8404 at 2µg/ml staining DAP kinase 2 in mouse spleen cells by IHC

References

ab8404 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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