Anti-DAP Kinase 2 antibody [EP1633Y] (ab51601)

Overview

  • Product nameAnti-DAP Kinase 2 antibody [EP1633Y]
    See all DAP Kinase 2 primary antibodies
  • Description
    Rabbit monoclonal [EP1633Y] to DAP Kinase 2
  • Specificityab51601 recognizes DAP Kinase 2.
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues on the N terminal of human DAP Kinase 2.

  • Positive control
    • WB: Rat spleen lysate IHC-P: Human liver carcinoma.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEP1633Y
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab51601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for ICC/IF or IP.
  • Target

    • FunctionCalcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.
    • Tissue specificityUbiquitously expressed in all tissue types examined. High levels in heart, lung and skeletal muscle.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
      Contains 1 protein kinase domain.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • DAK2 antibody
      • DAP kinase 2 antibody
      • DAP kinase related protein 1 antibody
      • DAP kinase related protein 1 beta isoform antibody
      • DAP-kinase-related protein 1 antibody
      • DAPK2 antibody
      • DAPK2_HUMAN antibody
      • Death associated protein kinase 2 antibody
      • Death-associated protein kinase 2 antibody
      • DRP 1 antibody
      • DRP-1 antibody
      • DRP1 antibody
      • MGC119312 antibody
      see all

    Anti-DAP Kinase 2 antibody [EP1633Y] images

    • Overlay histogram showing HeLa cells stained with ab51601 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51601, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • Anti-DAP Kinase 2 antibody [EP1633Y] (ab51601) at 1/2000 dilution + Rat spleen lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 42 kDa
      Observed band size : 42 kDa
    • Immunohistochemical staining of paraffin embedded human liver carcinoma using ab51601 (1/50). Secondary stained using Goat anti-rabbit HRP (1/2000).

    References for Anti-DAP Kinase 2 antibody [EP1633Y] (ab51601)

    This product has been referenced in:
    • Ber Y  et al. DAPK2 is a novel regulator of mTORC1 activity and autophagy. Cell Death Differ 22:465-75 (2015). Read more (PubMed: 25361081) »
    • Shoval Y  et al. New Modularity of DAP-Kinases: Alternative Splicing of the DRP-1 Gene Produces a ZIPk-Like Isoform. PLoS One 6:e17344 (2011). WB ; Human . Read more (PubMed: 21408167) »

    See all 2 Publications for this product

    Product Wall

    Thank you for contacting us with your questions, and I apologize for any confusion. Since ab51601 is not a purified antibody, we are unable to provide an exact concentration for this product. We recommend using the antibody at the dilutions on the data...

    Read More
    Application Western blot
    Sample African Green Monkey Cell lysate - whole cell (Various cells types (HeLa, 293T, MEFs, COS))
    Loading amount 60 µg
    Specification Various cells types (HeLa, 293T, MEFs, COS)
    Gel Running Conditions Non-reduced Denaturing (10% SDS page gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Username

    Mr. Yishay Shoval

    Verified customer

    Submitted Feb 25 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"