Anti-DAP1 antibody [E59] (ab32056)

Overview

  • Product nameAnti-DAP1 antibody [E59]
    See all DAP1 primary antibodies
  • Description
    Rabbit monoclonal [E59] to DAP1
  • Tested applicationsSuitable for: WB, IHC-P, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (C terminal)

  • Positive control
    • NIH3T3 cell lysate, Hela cell lysate and human stomach carcinoma
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab32056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Detects a band of approximately 11 kDa.
IHC-P 1/100 - 1/250.
IP 1/50.
ICC/IF 1/250.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    Anti-DAP1 antibody [E59] images

    • Anti-DAP1 antibody [E59] (ab32056) at 1/10000 dilution + NIH3T3 cell lysate

      Observed band size : 11 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 23 kDa. We are unsure as to the identity of these extra bands.
    • Immunofluorescent analysis of DAP1 expression in HeLa cells using 1/250 ab32056.
    • ab32056, at a 1/100 dilution, staining human stomach carcinoma by immunohistochemistry, Paraffin embedded tissue.

    References for Anti-DAP1 antibody [E59] (ab32056)

    ab32056 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your reply. Unfortunately, I am not an expert on DAP1 so I don't have any further information about what effect the acetylserine has in the cell. According to the uniprot database, the reference PMID 16602700 contains additional informat...

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    Thank you for contacting us. We do not currently sell an antibody to DAP1 that is specific for the N-acetylserine modification. If you are looking to detect this modification, you may be able to perform an IP for the total DAP1 protein using ab32056 ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"