Overview

  • Product name
  • Description
    Goat polyclonal to DAP12
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Sheep, Dog
  • Immunogen

    Synthetic peptide:

    KQRITETESPYQE

    , corresponding to amino acids 81-93 of Human DAP12

  • Positive control
    • Human Spleen and Human Uterus tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab110117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation.
  • Tissue specificity
    Expressed at low levels in the early development of the hematopoietic system and in the promonocytic stage and at high levels in mature monocytes. Expressed in hematological cells and tissues such as peripheral blood leukocytes and spleen. Also found in bone marrow, lymph nodes, placenta, lung and liver. Expressed at lower levels in different parts of the brain especially in the basal ganglia and corpus callosum.
  • Involvement in disease
    Defects in TYROBP are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also called presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns.
  • Sequence similarities
    Belongs to the TYROBP family.
  • Post-translational
    modifications
    Tyrosine phosphorylated.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DAP 12 antibody
    • DAP12 antibody
    • DNAX activation protein 12 antibody
    • DNAX-activation protein 12 antibody
    • KAR-associated protein antibody
    • KARAP antibody
    • Killer activating receptor associated protein antibody
    • Killer-activating receptor-associated protein antibody
    • PLOSL antibody
    • TYOBP_HUMAN antibody
    • TYRO protein tyrosine kinase binding protein antibody
    • TYRO protein tyrosine kinase-binding protein antibody
    • TYROBP antibody
    see all

Images

  • Human Spleen (formalin-fixed, paraffin-embedded) stained with 3 µg/ml ab110117 followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Human Uterus (formalin-fixed, paraffin-embedded) stained with 3 µg/ml ab110117 followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References

ab110117 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10%
Sample
Human Tissue sections (Human brain section)
Specification
Human brain section
Permeabilization
Yes - 0.1% TritonX in 0.1% PBS
Fixative
Formaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Aug 06 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up