Anti-DAP12 antibody (ab93846)
Key features and details
- Rabbit polyclonal to DAP12
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DAP12 antibody
See all DAP12 primary antibodies -
Description
Rabbit polyclonal to DAP12 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Sheep, Cow, Dog -
Immunogen
Synthetic peptide corresponding to Human DAP12 aa 50 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab107906) -
Positive control
- This antibody gave a positive signal in the follwoing lysates: Lung (Human) Tissue Lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93846 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa). |
Target
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Function
Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation. -
Tissue specificity
Expressed at low levels in the early development of the hematopoietic system and in the promonocytic stage and at high levels in mature monocytes. Expressed in hematological cells and tissues such as peripheral blood leukocytes and spleen. Also found in bone marrow, lymph nodes, placenta, lung and liver. Expressed at lower levels in different parts of the brain especially in the basal ganglia and corpus callosum. -
Involvement in disease
Defects in TYROBP are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also called presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. -
Sequence similarities
Belongs to the TYROBP family. -
Post-translational
modificationsTyrosine phosphorylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 282390 Cow
- Entrez Gene: 476477 Dog
- Entrez Gene: 7305 Human
- Entrez Gene: 443208 Sheep
- Omim: 604142 Human
- SwissProt: Q95J79 Cow
- SwissProt: O43914 Human
- Unigene: 515369 Human
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Alternative names
- DAP 12 antibody
- DAP12 antibody
- DNAX activation protein 12 antibody
see all
Images
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All lanes : Anti-DAP12 antibody (ab93846) at 1 µg/ml
Lane 1 : Wild-type THP-1 cell lysate
Lane 2 : TYROBP CRISPR-Cas9 edited THP-1 cell lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 12 kDa
Observed band size: 12 kDaFalse colour image of Western blot: Anti-DAP12 antibody staining at 1 ug/ml, shown in black; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab93846 was shown to bind specifically to DAP12. A band was observed at 12 kDa in wild-type THP-1 cell lysates with no signal observed at this size in TYROBP CRISPR-Cas9 edited cell line ab273726 (CRISPR-Cas9 edited cell lysate ab273774). The band observed in the CRISPR-Cas9 edited lysate lane below 12 kDa is likely to represent a truncated form of DAP12. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and TYROBP CRISPR-Cas9 edited THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % BSA in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent ab133456) and imaged with 2 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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Anti-DAP12 antibody (ab93846) at 1 µg/ml + Lung (Human) Tissue Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 12 kDa
Observed band size: 12 kDa
Additional bands at: 200 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
The DAP12 protein sequence contains a 21-residue signal sequence at the N-terminus. Cleavage of this peptide may account for the doublet observed.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab93846 has been referenced in 1 publication.
- Raha AA et al. Neuroprotective Effect of TREM-2 in Aging and Alzheimer's Disease Model. J Alzheimers Dis N/A:N/A (2016). IHC ; Mouse . PubMed: 27662313