Recombinant Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15867-28] to DAP13/NDUFA12
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-DAP13/NDUFA12 antibody [EPR15867-28]
See all DAP13/NDUFA12 primary antibodies -
Description
Rabbit monoclonal [EPR15867-28] to DAP13/NDUFA12 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa cells; Human fetal heart and skeletal muscle tissue lysates; Mouse brain, heart and kidney tissue lysates; Rat muscle tissue lysate; Human brain and stomach tissues; Rat kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15867-28 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192617 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/2000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
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IHC-P |
1/400 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/100.
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Notes |
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WB
1/1000 - 1/2000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). |
IHC-P
1/400 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/100. |
Target
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Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. -
Involvement in disease
Defects in NDUFA12 are the cause of Leigh syndrome (LS) [MIM:256000]. An early-onset progressive neurodegenerative disorder characterized by the presence of focal, bilateral lesions in one or more areas of the central nervous system including the brainstem, thalamus, basal ganglia, cerebellum and spinal cord. Clinical features depend on which areas of the central nervous system are involved and include subacute onset of psychomotor retardation, hypotonia, ataxia, weakness, vision loss, eye movement abnormalities, seizures, and dysphagia. -
Sequence similarities
Belongs to the complex I NDUFA12 subunit family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 55967 Human
- Entrez Gene: 66414 Mouse
- Entrez Gene: 299739 Rat
- Omim: 614530 Human
- SwissProt: Q9UI09 Human
- SwissProt: Q7TMF3 Mouse
- Unigene: 506374 Human
- Unigene: 27886 Mouse
see all -
Alternative names
- 13 kDa differentiation associated protein antibody
- 13 kDa differentiation-associated protein antibody
- 2410011G03Rik antibody
see all
Images
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All lanes : Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617) at 1/1000 dilution
Lane 1 : mouse brain tissue lysate
Lanes 2 & 4 : mouse heart tissue lysate
Lanes 3 & 5 : mouse kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 17 kDa -
Immunofluorescent analysis of HeLa cells (100% Methanol-fixed, 0.1% triton X-100-permealized) labeling DAP13/NDUFA12 with ab192617 at 1/100 dilution (20 μg/ml). A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with Counterstain: DAPI.
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Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617) at 1/5000 dilution + Rat muscle tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 17 kDa -
Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617) at 1/5000 dilution + Human fetal heart tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 17 kDa -
All lanes : Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617) at 1/1000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : Human skeletal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 17 kDa -
Immunohistochemical analysis of paraffin-embedded Rat muscle tissue labeling DAP13/NDUFA12 with ab192617 at 1/1000 dilution (2μg/ml). A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling DAP13/NDUFA12 with ab192617 at 1/1000 dilution (2μg/ml). A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling DAP13/NDUFA12 with ab192617 at 1/1000 dilution (2μg/ml). A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab192617 has been referenced in 3 publications.
- Ahn M et al. A requirement for PAK1 to support mitochondrial function and maintain cellular redox balance via electron transport chain proteins to prevent ß-cell apoptosis. Metabolism 115:154431 (2021). PubMed: 33181191
- Rao S et al. AIF-regulated oxidative phosphorylation supports lung cancer development. Cell Res 29:579-591 (2019). PubMed: 31133695
- Li HB et al. Mitochondrial Ribosomal Protein L10 Associates with Cyclin B1/Cdk1 Activity and Mitochondrial Function. DNA Cell Biol 35:680-690 (2016). WB ; Human . PubMed: 27726420