Overview

  • Product name
    Anti-DAP13 antibody [EPR15867-28]
    See all DAP13 primary antibodies
  • Description
    Rabbit monoclonal [EPR15867-28] to DAP13
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human DAP13 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9UI09

  • Positive control
    • HeLa cells; Human fetal heart and skeletal muscle tissue lysates; Mouse brain, heart and kidney tissue lysates; Rat muscle tissue lysate; Human brain and stomach tissues; Rat kidney tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab192617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IHC-P 1/400 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100.

Target

  • Function
    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Involvement in disease
    Defects in NDUFA12 are the cause of Leigh syndrome (LS) [MIM:256000]. An early-onset progressive neurodegenerative disorder characterized by the presence of focal, bilateral lesions in one or more areas of the central nervous system including the brainstem, thalamus, basal ganglia, cerebellum and spinal cord. Clinical features depend on which areas of the central nervous system are involved and include subacute onset of psychomotor retardation, hypotonia, ataxia, weakness, vision loss, eye movement abnormalities, seizures, and dysphagia.
  • Sequence similarities
    Belongs to the complex I NDUFA12 subunit family.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 13 kDa differentiation associated protein antibody
    • 13 kDa differentiation-associated protein antibody
    • 2410011G03Rik antibody
    • AW112974 antibody
    • B17.2 antibody
    • CI-B17.2 antibody
    • CIB17.2 antibody
    • Complex I B17.2 antibody
    • Complex I B17.2 subunit antibody
    • Complex I-B17.2 antibody
    • DAP13 antibody
    • MGC107642 antibody
    • MGC7999 antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 12 antibody
    • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 antibody
    • NADH ubiquinone oxidoreductase subunit B17.2 antibody
    • NADH-ubiquinone oxidoreductase subunit B17.2 antibody
    • NDUAC_HUMAN antibody
    • NDUFA12 antibody
    • RGD1311462 antibody
    see all

Images

  • All lanes : Anti-DAP13 antibody [EPR15867-28] (ab192617) at 1/1000 dilution

    Lane 1 : mouse brain tissue lysate
    Lane 2 : mouse heart tissue lysate
    Lane 3 : mouse kidney tissue lysate
    Lane 4 : mouse heart tissue lysate
    Lane 5 : mouse kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size : 17 kDa
  • Immunofluorescent analysis of HeLa cells (100% Methanol-fixed, 0.1% triton X-100-permealized) labeling DAP13 with ab192617 at 1/100 dilution (20 μg/ml). A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with Counterstain: DAPI.

  • Anti-DAP13 antibody [EPR15867-28] (ab192617) at 1/5000 dilution + Rat muscle tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size : 17 kDa
  • Anti-DAP13 antibody [EPR15867-28] (ab192617) at 1/5000 dilution + Human fetal heart tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size : 17 kDa
  • All lanes : Anti-DAP13 antibody [EPR15867-28] (ab192617) at 1/1000 dilution

    Lane 1 : Human fetal heart tissue lysate
    Lane 2 : Human skeletal muscle tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size : 17 kDa
  • Immunohistochemical analysis of paraffin-embedded Rat muscle tissue labeling DAP13 with ab192617 at 1/1000 dilution (2μg/ml). A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling DAP13 with ab192617 at 1/1000 dilution (2μg/ml). A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling DAP13 with ab192617 at 1/1000 dilution (2μg/ml). A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.

References

This product has been referenced in:
  • Li HB  et al. Mitochondrial Ribosomal Protein L10 Associates with Cyclin B1/Cdk1 Activity and Mitochondrial Function. DNA Cell Biol 35:680-690 (2016). WB ; Human . Read more (PubMed: 27726420) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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