Recombinant
RabMAb

Anti-DAP13 antibody [EPR15867] (ab192607)

Overview

  • Product name
    Anti-DAP13 antibody [EPR15867]
    See all DAP13 primary antibodies
  • Description
    Rabbit monoclonal [EPR15867] to DAP13
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human DAP13 aa 1 to the C-terminus.
    Database link: Q9UI09

  • Positive control
    • Human fetal heart, fetal skeletal muscle, fetal kidney, fetal liver lysates. Mouse brain, rat brain, rat muscle lysates. Human pancreas tissue, mouse liver tissue. Hela cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab192607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 17,7 kDa.
IHC-P 1/300 - 1/600. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/50.

Target

  • Function
    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Involvement in disease
    Defects in NDUFA12 are the cause of Leigh syndrome (LS) [MIM:256000]. An early-onset progressive neurodegenerative disorder characterized by the presence of focal, bilateral lesions in one or more areas of the central nervous system including the brainstem, thalamus, basal ganglia, cerebellum and spinal cord. Clinical features depend on which areas of the central nervous system are involved and include subacute onset of psychomotor retardation, hypotonia, ataxia, weakness, vision loss, eye movement abnormalities, seizures, and dysphagia.
  • Sequence similarities
    Belongs to the complex I NDUFA12 subunit family.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 13 kDa differentiation associated protein antibody
    • 13 kDa differentiation-associated protein antibody
    • 2410011G03Rik antibody
    • AW112974 antibody
    • B17.2 antibody
    • CI-B17.2 antibody
    • CIB17.2 antibody
    • Complex I B17.2 antibody
    • Complex I B17.2 subunit antibody
    • Complex I-B17.2 antibody
    • DAP13 antibody
    • MGC107642 antibody
    • MGC7999 antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 12 antibody
    • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 antibody
    • NADH ubiquinone oxidoreductase subunit B17.2 antibody
    • NADH-ubiquinone oxidoreductase subunit B17.2 antibody
    • NDUAC_HUMAN antibody
    • NDUFA12 antibody
    • RGD1311462 antibody
    see all

Images

  • Immunofluorescent analysis of Hela cells permeabilised in 0.1% Triton X-100 and fixed in 4% paraformaldehyde labeling DAP13 with ab192607 at 1/50 (5.9 μg/ml) and Goat anti rabbit IgG(Alexa Fluor® 488) ab150077. Counterstained with DAPI.

  • All lanes : Anti-DAP13 antibody [EPR15867] (ab192607) at 1/1000 dilution

    Lane 1 : Human fetal heart
    Lane 2 : Human skeletal muscle

    Lysates/proteins at 20 µg per lane.

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size : 17,7 kDa
  • All lanes : Anti-DAP13 antibody [EPR15867] (ab192607) at 1/5000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 17,7 kDa
    Additional bands at : 7 kDa (possible isoform).
  • Anti-DAP13 antibody [EPR15867] (ab192607) at 1/5000 dilution + Rat muscle at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 17,7 kDa
  • All lanes : Anti-DAP13 antibody [EPR15867] (ab192607) at 1/1000 dilution

    Lane 1 : Mouse brain
    Lane 2 : Rat brain

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 17,7 kDa
  • Immunohistochemical analysis of formalin fixed paraffin embedded Human pancreas labeling DAP13 with ab192607 at 1/600 (0.5μg/ml) dilution and prediluted HRP polymer to Rabbit/Mouse IgG as secondary.

  • Immunohistochemical analysis of formalin fixed paraffin embedded mouse liver labeling DAP13 with ab192607 at 1/600 (0.5μg/ml) dilution and prediluted HRP polymer to Rabbit/Mouse IgG as secondary.

References

ab192607 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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