Overview

  • Product name
  • Description
    Goat polyclonal to DAP3
  • Specificity
    This antibody is expected to recognise both human isoforms of this protein.
  • Tested applications
    Suitable for: ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    NPSLLERHCAYL

    , corresponding to C terminal amino acids 387-398 of Human DAP3.

  • Positive control
    • HeLa lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab2637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 3 - 10 µg/ml.
ELISA 1/16000.
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 46 kDa.Can be blocked with Human DAP3 peptide (ab22922).

Target

  • Function
    Involved in mediating interferon-gamma-induced cell death.
  • Tissue specificity
    Ubiquitous.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • 28S ribosomal protein S29 antibody
    • 28S ribosomal protein S29 mitochondrial antibody
    • bMRP10 antibody
    • DAP 3 antibody
    • DAP-3 antibody
    • Dap3 antibody
    • Death associated protein 3 antibody
    • Death-associated protein 3 antibody
    • DKFZp686G12159 antibody
    • FLJ12817 antibody
    • Ionizing radiation resistance conferring protein antibody
    • MGC126058 antibody
    • MGC126059 antibody
    • Mitochondrial 28S ribosomal protein S29 antibody
    • mitochondrial antibody
    • MITOCHONDRIAL RIBOSOMAL PROTEIN S29 antibody
    • MRP S29 antibody
    • MRP-S29 antibody
    • MRPS29 antibody
    • OTTHUMP00000033480 antibody
    • OTTHUMP00000033481 antibody
    • PSD-95/SAP90-binding protein 3 antibody
    • RT29_HUMAN antibody
    • S29mt antibody
    • SAP90/PSD-95-associated protein 3 antibody
    • SAPAP3 antibody
    see all

Images

  • ab2637 staining DAP3 in HeLa cells by Immunocytochemistry/ Immunofluorescence before(left) and after(right) si-RNA-mediateded DAP3 knockdown expression. ab2637 diluted to 0.5 μg/ml and incubated at room temperature for 1 hour. Nuclei stained with DAPI.

  • All lanes : Anti-DAP3 antibody (ab2637) at 1 µg/ml (for 1 hour)

    Lane 1 : HeLa cell lysate in RIPA buffer - control
    Lane 2 : HeLa cell lysate in RIPA buffer - after siRNA mediated DAP3 knock-down expression

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 46 kDa

    Level of knock-down relative to actin expression level determined by RT-PCR.

  • All lanes : Anti-DAP3 antibody (ab2637)

    Lane 1 : HEK293 mock transfection cell lysate
    Lane 2 : HEK293 overexpressing DAP3 cell lysate


    Predicted band size : 46 kDa
  • Anti-DAP3 antibody (ab2637) at 3 µg/ml (for 1 hour) + HeLa cell lysate in RIPA buffer at 30 µg

    Predicted band size : 46 kDa

References

ab2637 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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