Overview

  • Product nameAnti-DAP5 antibody
    See all DAP5 primary antibodies
  • Description
    Rabbit polyclonal to DAP5
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 144-193 (PNFDGPAAEG QPGQKQSTTF RRLLISKLQD EFENRTRNVD VYDKRENPLL) of human DAP5 (NP_001409).

  • Positive control
    • 721_B cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 102 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

Target

  • FunctionAppears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases.
  • Tissue specificityUbiquitously expressed in all adult tissues examined, with high levels in skeletal muscle and heart. Also expressed in fetal brain, lung, liver and kidney.
  • Sequence similaritiesBelongs to the eIF4G family.
    Contains 1 MI domain.
    Contains 1 MIF4G domain.
    Contains 1 W2 domain.
  • Post-translational
    modifications
    Phosphorylation; hyperphosphorylated during mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • DAP-5 antibody
    • Death associated protein 5 antibody
    • Death-associated protein 5 antibody
    • eIF-4-gamma 2 antibody
    • eIF-4G 2 antibody
    • eIF4G 2 antibody
    • EIF4G2 antibody
    • Eukaryotic translation initiation factor 4 gamma 2 antibody
    • IF4G2_HUMAN antibody
    • Nat1 antibody
    • Novel APOBEC-1 target 1 antibody
    • p97 antibody
    • Translation repressor NAT1 antibody
    • Translation repressor NAT1 antibody
    see all

Anti-DAP5 antibody images

  • Anti-DAP5 antibody (ab83358) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 102 kDa
    Observed band size : 135 kDa (why is the actual band size different from the predicted?)

References for Anti-DAP5 antibody (ab83358)

ab83358 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"