Overview

  • Product name
  • Description
    Rabbit polyclonal to DAP5
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
  • Immunogen

    Synthetic peptide corresponding to a region between residues 800 and 850 of Human DAP5 (NP_001409.1)

  • Positive control
    • HeLa cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab85963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 5 - 10 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 102 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases.
    • Tissue specificity
      Ubiquitously expressed in all adult tissues examined, with high levels in skeletal muscle and heart. Also expressed in fetal brain, lung, liver and kidney.
    • Sequence similarities
      Belongs to the eIF4G family.
      Contains 1 MI domain.
      Contains 1 MIF4G domain.
      Contains 1 W2 domain.
    • Post-translational
      modifications
      Phosphorylation; hyperphosphorylated during mitosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • DAP-5 antibody
      • Death associated protein 5 antibody
      • Death-associated protein 5 antibody
      • eIF-4-gamma 2 antibody
      • eIF-4G 2 antibody
      • eIF4G 2 antibody
      • EIF4G2 antibody
      • Eukaryotic translation initiation factor 4 gamma 2 antibody
      • IF4G2_HUMAN antibody
      • Nat1 antibody
      • Novel APOBEC-1 target 1 antibody
      • p97 antibody
      • Translation repressor NAT1 antibody
      • Translation repressor NAT1 antibody
      see all

    Images

    • All lanes : Anti-DAP5 antibody (ab85963) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique

      Predicted band size : 102 kDa


      Exposure time : 3 minutes
    • 1mg HeLa whole cell lysate was immunoprecipitated using 10µg ab85963.
      20% of IP was loaded per lane and probed with ab85963 at 0.4µg/ml (lane 1) and a control IgG (lane 2).
      Detection: chemiluminescence with an exposure time of 30 seconds.

    References

    This product has been referenced in:
    • Xie JD  et al. Presynaptic N-Methyl-d-aspartate (NMDA) Receptor Activity Is Increased Through Protein Kinase C in Paclitaxel-induced Neuropathic Pain. J Biol Chem 291:19364-73 (2016). Read more (PubMed: 27458019) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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