Anti-P97/DAP5 antibody (ab97302)
Key features and details
- Rabbit polyclonal to P97/DAP5
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-P97/DAP5 antibody
See all P97/DAP5 primary antibodies -
Description
Rabbit polyclonal to P97/DAP5 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Cow -
Immunogen
Recombinant fragment corresponding to Human P97/DAP5 aa 1-204.
Database link: P78344 -
Positive control
- WB: A431 whole cell lysate. Mouse brain whole cell lysate. IHC-P: Cal27 xenograft, human skeletal muscle. Mouse hind brain tissue. IF/ICC: HeLa cell line.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 59.99% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97302 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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WB | (2) |
1/500 - 1/3000. Predicted molecular weight: 102 kDa.
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IHC-P |
1/100 - 1/1000.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/500 - 1/3000. Predicted molecular weight: 102 kDa. |
IHC-P
1/100 - 1/1000. |
Target
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Function
Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases. -
Tissue specificity
Ubiquitously expressed in all adult tissues examined, with high levels in skeletal muscle and heart. Also expressed in fetal brain, lung, liver and kidney. -
Sequence similarities
Belongs to the eIF4G family.
Contains 1 MI domain.
Contains 1 MIF4G domain.
Contains 1 W2 domain. -
Post-translational
modificationsPhosphorylation; hyperphosphorylated during mitosis. - Information by UniProt
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Database links
- Entrez Gene: 286870 Cow
- Entrez Gene: 1982 Human
- Entrez Gene: 13690 Mouse
- Entrez Gene: 361628 Rat
- Omim: 602325 Human
- SwissProt: P78344 Human
- SwissProt: Q62448 Mouse
- SwissProt: P79398 Rabbit
see all -
Alternative names
- DAP-5 antibody
- DAP5 antibody
- Death associated protein 5 antibody
see all
Images
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Anti-P97/DAP5 antibody (ab97302) at 1/500 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 102 kDa5% SDS PAGE
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Immunohistochemical analysis of paraffin-embedded Cal27 xenograft using ab97302 at 1/100 dilution
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HeLa cells stained for EIF4G2 (green) using ab97302 at 1/500 dilution in ICC/IF.
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Paraffin embedded mouse hind brain tissue stained for DAP5 using ab97302 at 1/500 dilution in immunohistochemical analysis.
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Anti-P97/DAP5 antibody (ab97302) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 102 kDa
7.5% SDS PAGE -
ICC/IF image of ab97302 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97302, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Paraffin embedded human skeletal muscle tissue stained for DAP5 using ab97302 at 10 μg/ml in immunohistochemical analysis.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab97302 has been referenced in 1 publication.
- Li S et al. MiR-144-3p-mediated dysregulation of EIF4G2 contributes to the development of hepatocellular carcinoma through the ERK pathway. J Exp Clin Cancer Res 40:53 (2021). PubMed: 33526055