For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Goat polyclonal to DAPP1
HumanPredicted to work with:
Peptide with sequence C-
, corresponding to C terminal amino acids 257-269 from the internal region (near C Terminus) of Human DAPP1 (NP_055210.2)
- Transfected HEK293 cell lysate
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).
May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K.
Highly expressed in placenta and lung, followed by brain, heart, kidney, liver, pancreas and skeletal muscle. Expressed by B-lymphocytes, but not T-lymphocytes or nonhematopoietic cells.
Contains 1 PH domain.
Contains 1 SH2 domain.
Phosphorylated on tyrosine residues.
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
Information by UniProt
- B cell adapter molecule of 32 kDa antibody
- B lymphocyte adapter protein Bam32 antibody
- B-cell adapter molecule of 32 kDa antibody
Western blot - Anti-DAPP1 antibody (ab123498)
Lane 1 :
Anti-DAPP1 antibody (ab123498) at 0.1 µg/mlLane 2 :
Anti-DAPP1 antibody (ab123498) at 1 mg/mlLane 3 :
Anti-DYKDDDDK Tag antibody at 1/3000 dilutionLane 1 :
HEK293 lysate in RIPA buffer over expressing Human DAPP1 with DYKDDDDK tag Lane 2 :
Mock-transfected HEK293 Lane 3 :
HEK293 lysate in RIPA buffer over expressing Human DAPP1 with DYKDDDDK tag Predicted band size :
32 kDaObserved band size :
35 kDa (why is the actual band size different from the predicted?
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"