Anti-DAPP1 (phospho Y139) antibody (ab63396)


  • Product nameAnti-DAPP1 (phospho Y139) antibody
    See all DAPP1 primary antibodies
  • Description
    Rabbit polyclonal to DAPP1 (phospho Y139)
  • Specificityab63396 detects endogenous levels of DAPP1 only when phosphorylated at tyrosine 139.
  • Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human DAPP1 around the phosphorylation site of tyrosine 139 (S-I-YP-E-S).

  • Positive control
    • WB: 293 cell extracts treated with Insulin (0.01U/ml, 2mins). ICC/IF: A549 cells.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab63396 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab63396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. Predicted molecular weight: 32 kDa.
ELISA 1/5000.


  • FunctionMay act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K.
  • Tissue specificityHighly expressed in placenta and lung, followed by brain, heart, kidney, liver, pancreas and skeletal muscle. Expressed by B-lymphocytes, but not T-lymphocytes or nonhematopoietic cells.
  • Sequence similaritiesContains 1 PH domain.
    Contains 1 SH2 domain.
  • Post-translational
    Phosphorylated on tyrosine residues.
  • Cellular localizationCytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
  • Information by UniProt
  • Database links
  • Alternative names
    • B cell adapter molecule of 32 kDa antibody
    • B lymphocyte adapter protein Bam32 antibody
    • B-cell adapter molecule of 32 kDa antibody
    • BAM32 antibody
    • DAPP1 antibody
    • DAPP1_HUMAN antibody
    • DKFZp667E0716 antibody
    • Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide antibody
    • Dual adaptor of phosphotyrosine and 3 phosphoinositides antibody
    • hDAPP1 antibody
    see all

Anti-DAPP1 (phospho Y139) antibody images

  • ab63396, at 1/500 dilution, staining DAPP1 in A549 cells by Immunofluorescence, in the absence (left image) or presence (right image) of the immunising peptide.
  • All lanes : Anti-DAPP1 (phospho Y139) antibody (ab63396) at 1/500 dilution

    Lane 1 : 293 cell extracts treated with Insulin (0.01U/ml, 2mins)
    Lane 2 : 293 cell extracts treated with Insulin (0.01U/ml, 2mins) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 32 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

References for Anti-DAPP1 (phospho Y139) antibody (ab63396)

ab63396 has not yet been referenced specifically in any publications.

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