For the best experience on the Abcam website please upgrade to a modern browser such as
Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab63396 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000.
1/500 - 1/1000. Predicted molecular weight: 32 kDa.
Function May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K.
Tissue specificity Highly expressed in placenta and lung, followed by brain, heart, kidney, liver, pancreas and skeletal muscle. Expressed by B-lymphocytes, but not T-lymphocytes or nonhematopoietic cells.
Sequence similarities Contains 1 PH domain. Contains 1 SH2 domain.
Post-translational modifications Phosphorylated on tyrosine residues.
Cellular localization Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
Information by UniProt
B cell adapter molecule of 32 kDa antibody
B lymphocyte adapter protein Bam32 antibody
B-cell adapter molecule of 32 kDa antibody
Anti-DAPP1 (phospho Y139) antibody images
Immunocytochemistry/ Immunofluorescence - DAPP1 (phospho Y139) antibody (ab63396)
ab63396, at 1/500 dilution, staining DAPP1 in A549 cells by Immunofluorescence, in the absence (left image) or presence (right image) of the immunising peptide.
Western blot - DAPP1 (phospho Y139) antibody (ab63396)
All lanes : Anti-DAPP1 (phospho Y139) antibody (ab63396) at 1/500 dilution Lane 1 : 293 cell extracts treated
with Insulin (0.01U/ml, 2mins) Lane 2 : 293 cell extracts treated
with Insulin (0.01U/ml, 2mins) with immunising peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size : 32 kDa Observed band size : 27 kDa ( why is the actual band size different from the predicted?) Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
References for Anti-DAPP1 (phospho Y139) antibody (ab63396)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"