Overview

  • Product nameAnti-DARC antibody
    See all DARC primary antibodies
  • Description
    Rabbit polyclonal to DARC
  • Tested applicationsSuitable for: ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide from the N terminus of Human DARC.

  • Positive control
    • Human thymus tissue; A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab124648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/40000.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF 1/100 - 1/500.

Target

  • FunctionNon-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi.
  • Tissue specificityFound in adult kidney, adult spleen, bone marrow and fetal liver. In particular, it is expressed along postcapillary venules throughout the body, except in the adult liver. Erythroid cells and postcapillary venule endothelium are the principle tissues expressing duffy. Fy(-A-B) individuals do not express duffy in the bone marrow, however they do, in postcapillary venule endothelium.
  • Sequence similaritiesBelongs to the G-protein coupled receptor Duffy family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCBP1 antibody
    • CD234 antibody
    • DARC antibody
    • Dfy antibody
    • Duffy antigen/chemokine receptor antibody
    • duffy blood group antibody
    • Duffy blood group antigen antibody
    • Duffy blood group chemokine receptor antibody
    • DUFFY_HUMAN antibody
    • FY antibody
    • Fy glycoprotein antibody
    • Glycoprotein D antibody
    • GPD antibody
    • GpFy antibody
    • Plasmodium vivax receptor antibody
    • WBCQ1 antibody
    see all

Anti-DARC antibody images

  • ab124648, at 5µg/ml, staining DARC in Formalin-fixed, Paraffin-embedded Human Thymus vessel tissue by Immunohistochemistry.
  • ab124648, at 1/100 dilution, staining DARC in A549 cells by Immunofluorescence. The image on the right is treated with the synthesized peptide.

References for Anti-DARC antibody (ab124648)

ab124648 has not yet been referenced specifically in any publications.

Product Wall



The immunogenic peptide does not overlap with the FY6 aa#19-25 region. That being said, the amino range of the immunogen is aa#30-60 of human DARC with acc#Q16570 (http://www.uniprot.org/uniprot/Q16570), and aa#42 is in that range.

Unfortunately, the lab will not release the sequence of the peptide used to generate this antibody. They did tell me that the immunogenic peptide does not overlap with the region described you have described.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"