Anti-DARC antibody (ab40821)

Overview

  • Product nameAnti-DARC antibody
    See all DARC primary antibodies
  • Description
    Goat polyclonal to DARC
  • Tested applicationsSuitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Hoolock Gibbon
  • Immunogen

    Synthetic peptide:

    C-HRAELSPSTENSSQLDFED

    , corresponding to N terminal amino acids 6-24 of Human DARC

  • Positive control
    • Human kidney and human liver lysates. Human kidney tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab40821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. (see Abreview for further details published on 3rd April, 2007).



ab37373-Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 3 µg/ml.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa).

Target

  • FunctionNon-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi.
  • Tissue specificityFound in adult kidney, adult spleen, bone marrow and fetal liver. In particular, it is expressed along postcapillary venules throughout the body, except in the adult liver. Erythroid cells and postcapillary venule endothelium are the principle tissues expressing duffy. Fy(-A-B) individuals do not express duffy in the bone marrow, however they do, in postcapillary venule endothelium.
  • Sequence similaritiesBelongs to the G-protein coupled receptor Duffy family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCBP1 antibody
    • CD234 antibody
    • DARC antibody
    • Dfy antibody
    • Duffy antigen/chemokine receptor antibody
    • duffy blood group antibody
    • Duffy blood group antigen antibody
    • Duffy blood group chemokine receptor antibody
    • DUFFY_HUMAN antibody
    • FY antibody
    • Fy glycoprotein antibody
    • Glycoprotein D antibody
    • GPD antibody
    • GpFy antibody
    • Plasmodium vivax receptor antibody
    • WBCQ1 antibody
    see all

Anti-DARC antibody images

  • Anti-DARC antibody (ab40821) at 0.1 µg/ml + Human liver lysate - (35µg protein in RIPA buffer).

    Secondary
    anti-goat-HRP at 1/3000 dilution

    Predicted band size : 36 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • ab40821 at 3µg/ml staining DARC in human kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Microwaved antigen retrieval using citrate buffer pH 6 was performed, HRP-staining.
    This antobody stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein. This antibody has also been successfully used on human lung tissue showing endothelial staining.

References for Anti-DARC antibody (ab40821)

ab40821 has not yet been referenced specifically in any publications.

Product Wall

The immunogen sequence for ab40821 does not include any cysteine or methionine, so there will not be any thiol formation or methionine oxidation to affect the epitopes the antibody recognizes. The immunogen for ab58965 is near the C terminus but we do ...

Read More
Application Western blot
Sample Human Cell lysate - whole cell (human colorectal cancer cell line HCT116)
Loading amount 50 µg
Specification human colorectal cancer cell line HCT116
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 06 2012

Thank you for your recent review of ab40821.
I would like to follow-up with you since the results with this antibody were so poor. Were there no bands at all on the blot, or were there any non-specific bands? Did you try any other concentrations o...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Hela)
Specification Hela
Preparation Cell harvesting/tissue preparation method: trypsin
Sample buffer: PBS 1X
Fixation no fixation
Permeabilization No
Gating Strategy without debris
Username

Abcam user community

Verified customer

Submitted Nov 06 2007

Application Flow Cytometry
Specification HEK293S-TetR transfected with DARC receptor
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Apr 03 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"