Overview

  • Product nameAnti-DARC antibody
    See all DARC primary antibodies
  • Description
    Rabbit polyclonal to DARC
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    A 16 amino acid peptide from near the carboxy terminus of human DARC.

  • Positive control
    • WB: Mouse brain tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab58965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).

Target

  • FunctionNon-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi.
  • Tissue specificityFound in adult kidney, adult spleen, bone marrow and fetal liver. In particular, it is expressed along postcapillary venules throughout the body, except in the adult liver. Erythroid cells and postcapillary venule endothelium are the principle tissues expressing duffy. Fy(-A-B) individuals do not express duffy in the bone marrow, however they do, in postcapillary venule endothelium.
  • Sequence similaritiesBelongs to the G-protein coupled receptor Duffy family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCBP1 antibody
    • CD234 antibody
    • DARC antibody
    • Dfy antibody
    • Duffy antigen/chemokine receptor antibody
    • duffy blood group antibody
    • Duffy blood group antigen antibody
    • Duffy blood group chemokine receptor antibody
    • DUFFY_HUMAN antibody
    • FY antibody
    • Fy glycoprotein antibody
    • Glycoprotein D antibody
    • GPD antibody
    • GpFy antibody
    • Plasmodium vivax receptor antibody
    • WBCQ1 antibody
    see all

Anti-DARC antibody images

  • Lane 1 : Anti-DARC antibody (ab58965) at 0.5 µg/ml
    Lane 2 : Anti-DARC antibody (ab58965) at 1 µg/ml
    Lane 3 : Anti-DARC antibody (ab58965) at 2 µg/ml

    Lane 1 : mouse brain tissue lysate
    Lane 2 : mouse brain tissue lysate
    Lane 3 : mouse brain tissue lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    Lanes 1 - 2 : Anti-rabbit IgG HRP at 1/1000 dilution
    Lane 3 : Anti-rabbit IgG HRP at 1/10000 dilution


    Predicted band size : 36 kDa
    Observed band size : 36 kDa
    Additional bands at : 23 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab58965 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58965, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-DARC antibody (ab58965)

This product has been referenced in:
  • Samaniego R  et al. Mesenchymal contribution to recruitment, infiltration, and positioning of leukocytes in human melanoma tissues. J Invest Dermatol 133:2255-64 (2013). Human . Read more (PubMed: 23446986) »
  • Wessels JM  et al. Expression of chemokine decoy receptors and their ligands at the porcine maternal-fetal interface. Immunol Cell Biol : (2010). Pig . Read more (PubMed: 20680026) »

See all 2 Publications for this product

Product Wall

The immunogen sequence for ab40821 does not include any cysteine or methionine, so there will not be any thiol formation or methionine oxidation to affect the epitopes the antibody recognizes. The immunogen for ab58965 is near the C terminus but we do ...

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DISCOUNT CODE: ***
Expiration date: October 19th, 2012

I am very pleased to hear you would like to accept our offer and test ab58965 in IHC-P. This code will give you: 1 free primary antibody before the expiration date. To redeem this of...

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Thank you very much for your inquiry. However, the peptide sequences used to produce this antibody is proprietary information and cannot be disclosed at this time. I am sorry that I cannot help you further in this matter but please let ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"