Overview

  • Product nameAnti-DARPP32 antibody
    See all DARPP32 primary antibodies
  • Description
    Rabbit polyclonal to DARPP32
  • SpecificityDetects endogenous levels of total DARPP-32 protein.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic non-phosphopeptide derived from human DARPP-32 around the phosphorylation site of threonine 75 (A-Y-TP-P-P)

  • Positive control
    • Extracts from 293 cells treated with EGF, human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab51139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/40000.

Target

  • FunctionInhibitor of protein-phosphatase 1.
  • Sequence similaritiesBelongs to the protein phosphatase inhibitor 1 family.
  • Post-translational
    modifications
    Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.
    Phosphorylation of Thr-34 is required for activity.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • DARPP 32 antibody
    • DARPP-32 antibody
    • Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody
    • Dopamine and cAMP regulated neuronal phosphoprotein antibody
    • Dopamine and cAMP regulated phosphoprotein antibody
    • Dopamine and cAMP regulated phosphoprotein DARPP 32 antibody
    • Dopamine and cAMP regulated phosphoprotein DARPP32 antibody
    • Dopamine- and cAMP-regulated neuronal phosphoprotein antibody
    • FLJ20940 antibody
    • IPPD antibody
    • Neuronal phosphoprotein DARPP 32 antibody
    • PPP1R1B antibody
    • PPR1B_HUMAN antibody
    • Protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody
    • Protein phosphatase 1 regulatory inhibitor subunit 1B antibody
    • Protein phosphatase 1 regulatory subunit 1B antibody
    see all

Anti-DARPP32 antibody images

  • All lanes : Anti-DARPP32 antibody (ab51139) at 1/500 dilution

    Lane 1 : extracts from 293 cells treated with EGF
    Lane 2 : extracts from 293 cells treated with EGF with immunizing peptide

    Lysates/proteins at 0.2 µg/ml per lane.


    Predicted band size : 32 kDa
    Observed band size : 32 kDa
  • Ab51139 at 1/50 dilution staining human brain tissue, with and without blocking peptide; paraffin embedded.

References for Anti-DARPP32 antibody (ab51139)

ab51139 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"