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Rabbit polyclonal to DARPP32
Detects endogenous levels of total DARPP-32 protein.
HumanPredicted to work with:
Synthetic non-phosphopeptide derived from human DARPP-32 around the phosphorylation site of threonine 75 (A-Y-TP-P-P)
- Extracts from 293 cells treated with EGF, human brain tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
Corresponding phospho antibody
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
||Use at an assay dependent concentration.
Inhibitor of protein-phosphatase 1.
Belongs to the protein phosphatase inhibitor 1 family.
Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.
Phosphorylation of Thr-34 is required for activity.
Information by UniProt
- DARPP 32 antibody
- DARPP-32 antibody
- Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody
Western blot - DARPP32 antibody (ab51139)
All lanes : Anti-DARPP32 antibody (ab51139) at 1/500 dilution
Lane 1 : extracts from 293 cells treated with
Lane 2 : extracts from 293 cells treated with
EGF with immunizing peptide
Lysates/proteins at 0.2 µg/ml per lane.
Predicted band size : 32 kDa
Observed band size : 32 kDa
Immunohistochemistry (Paraffin-embedded sections) - DARPP32 antibody (ab51139)
Ab51139 at 1/50 dilution staining human brain tissue, with and without blocking peptide; paraffin embedded.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"