Anti-DARPP32 (phospho T75) antibody (ab18552)

Overview

  • Product nameAnti-DARPP32 (phospho T75) antibody
    See all DARPP32 primary antibodies
  • Description
    Rabbit polyclonal to DARPP32 (phospho T75)
  • SpecificityDetects endogenous levels of DARPP32 only when phosphorylated at threonine 75. Does not cross react with DARPP32 phosphorylated at Thr34 or non-phosphorylated DARPP32.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic phosphopeptide (Human) conjugated to KLH corresponding to residues surrounding T75 of DARPP32.

  • Positive control
    • rat brain cortex phosphorylated in vitro with cdc2

Properties

Applications

Our Abpromise guarantee covers the use of ab18552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: Use at an assay dependent dilution.
    WB: 1/1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.Predicted molecular weight: 32 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInhibitor of protein-phosphatase 1.
    • Sequence similaritiesBelongs to the protein phosphatase inhibitor 1 family.
    • Post-translational
      modifications
      Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.
      Phosphorylation of Thr-34 is required for activity.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • DARPP 32 antibody
      • DARPP-32 antibody
      • Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody
      • Dopamine and cAMP regulated neuronal phosphoprotein antibody
      • Dopamine and cAMP regulated phosphoprotein antibody
      • Dopamine and cAMP regulated phosphoprotein DARPP 32 antibody
      • Dopamine and cAMP regulated phosphoprotein DARPP32 antibody
      • Dopamine- and cAMP-regulated neuronal phosphoprotein antibody
      • FLJ20940 antibody
      • IPPD antibody
      • Neuronal phosphoprotein DARPP 32 antibody
      • PPP1R1B antibody
      • PPR1B_HUMAN antibody
      • Protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody
      • Protein phosphatase 1 regulatory inhibitor subunit 1B antibody
      • Protein phosphatase 1 regulatory subunit 1B antibody
      see all

    References for Anti-DARPP32 (phospho T75) antibody (ab18552)

    ab18552 has not yet been referenced specifically in any publications.

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