The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution.
WB: 1/1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.Predicted molecular weight: 32 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionInhibitor of protein-phosphatase 1.
Sequence similaritiesBelongs to the protein phosphatase inhibitor 1 family.
Post-translational modificationsDopamine- and cyclic AMP-regulated neuronal phosphoprotein. Phosphorylation of Thr-34 is required for activity.