Overview

  • Product name
    Anti-DARS antibody - C-terminal
    See all DARS primary antibodies
  • Description
    Rabbit polyclonal to DARS - C-terminal
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the C terminal region of human DARS conjugated to KLH

  • Positive control
    • K562 cell line lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    ab71436 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50.
ELISA 1/1000.
WB 1/50 - 1/100. Detects a band of approximately 55 kDa (predicted molecular weight: 57 kDa).

Target

  • Function
    Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.
  • Sequence similarities
    Belongs to the class-II aminoacyl-tRNA synthetase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Aspartate tRNA ligase 1 cytoplasmic antibody
    • Aspartate tRNA ligase antibody
    • Aspartate--tRNA ligase antibody
    • Aspartyl tRNA synthetase antibody
    • Aspartyl tRNA synthetase cytoplasmic antibody
    • Aspartyl-tRNA synthetase antibody
    • AspRS antibody
    • Cell proliferation inducing gene 40 protein antibody
    • Cell proliferation inducing protein 40 antibody
    • Cell proliferation-inducing gene 40 protein antibody
    • cytoplasmic antibody
    • Dars antibody
    • DKFZp781B11202 antibody
    • MGC111579 antibody
    • SYDC_HUMAN antibody
    see all

Images

  • Anti-DARS antibody - C-terminal (ab71436) at 1/60 dilution + K562 cell line lysate at 35 µg

    Predicted band size : 57 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • ab71436 at 1/50 dilution staining DARS C - terminal in human hepatocarcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

References

ab71436 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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