Overview

  • Product nameAnti-DARS2 antibody
    See all DARS2 primary antibodies
  • Description
    Mouse polyclonal to DARS2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human DARS2 protein (NP_060592.2).

  • Positive control
    • DARS2 transfected 293T lysate and human placenta tissue lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab69336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).

Target

  • RelevanceAspartyl-tRNA synthetase (DARS) charges its cognate tRNA with aspartate during protein biosynthesis. Mammalian synthetases differ from those of prokaryotes and lower eukaryotes in that they associate as multienzyme complexes. Defects in DARS2 are a cause of leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation.
  • Cellular localizationMitochondrion matrix.
  • Database links
  • Alternative names
    • Aspartate tRNA ligase 2 mitochondrial antibody
    • Aspartate tRNA ligase antibody
    • Aspartate tRNA ligase mitochondrial antibody
    • Aspartyl tRNA synthetase 2 (mitochondrial) antibody
    • Aspartyl tRNA synthetase mitochondrial antibody
    • AspRS antibody
    • FLJ10514 antibody
    • LBSL antibody
    • MT ASPRS antibody
    • RP3 383J4.2 antibody
    see all

Anti-DARS2 antibody images

  • Anti-DARS2 antibody (ab69336) at 1/500 dilution + Human placenta tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 74 kDa
    Observed band size : 74 kDa
  • All lanes : Anti-DARS2 antibody (ab69336) at 1/500 dilution

    Lane 1 : DARS2 transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 74 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

References for Anti-DARS2 antibody (ab69336)

ab69336 has not yet been referenced specifically in any publications.

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