Daudi whole cell lysate (ab3951)

Overview

  • Product nameDaudi whole cell lysate
  • General notesCell line: Daudi (Human Burkitt's lymphoma).
    Growth media: RPMI 1640 medium with 2 mM L-glutamine, 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate, and 10% fetal bovine serum.


    Daudi cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • Tested applicationsSuitable for: WBmore details

Properties

  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • Storage bufferConstituent: 5% Beta mercaptoethanol
  • Concentration information loading...
  • PurityWhole Cell Lysate
  • Lysate notesDaudi cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
  • Research areas
  • BackgroundDaudi cells are lymphoblast-like in morphology and contain the Epstein-Barr virus. They are positive for the Fc receptor and certain tumor markers and are used for studies of differentiation and tumorigenicity and for antitumour testing. The lysate is a positive control for: IKKalpha (numerous ab in our catalogue for IKK alpha), IKKbeta (=IKK2) (numerous ab in our catalogue for IKK beta), DR4 (numerous ab in our catalogue for DR4), NFkB p65 (numerous ab in our catalogue for NFkB p65).

Applications

Our Abpromise guarantee covers the use of ab3951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Daudi cell lysate is ready to load on SDS-PAGE for Western blotting, 10 - 20 µg per lane is recommended for mini gel.

References for Daudi whole cell lysate (ab3951)

ab3951 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"