Overview

  • Product nameAnti-DAZ1 antibody [3G10]
    See all DAZ1 primary antibodies
  • Description
    Mouse monoclonal [3G10] to DAZ1
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fusion protein corresponding to amino acids ~21 to ~121 of Human DAZ1 protein with a proprietary tag.

  • Positive control
    • Human liver and testis tissues; Human pancreas lysates; HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab115548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 83 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • RelevanceDAZ1 (deleted in azoospermia 1) is an RNA-binding protein that is essential in spermatogenesis. It may regulate translation of mRNAs by binding to the 3'-UTR.
  • Cellular localizationCytoplasmic and Nuclear. Note: Predominantly cytoplasmic. Nuclear at some stages of spermatozoide development. Localizes both to the nuclei and cytoplasm of spermatozoide differentiation. Nuclear in fetal gonocytes and in spermatogonial nuclei. It then relocates to the cytoplasm during male meiosis.
  • Database links
  • Alternative names
    • DAZ 1 antibody
    • DAZ antibody
    • Deleted in azoospermia 1 antibody
    • Deleted in azoospermia antibody
    • Deleted in azoospermia protein 1 antibody
    • SPGY antibody
    see all

Anti-DAZ1 antibody [3G10] images

  • ab115548, at 5 µg/ml, staining DAZ1 in formalin fixed, paraffin embedded Human liver tissue by Immunohistochemistry.
  • ab115548, at 5 µg/ml, staining DAZ1 in formalin fixed, paraffin embedded Human testis tissue by Immunohistochemistry.
  • Anti-DAZ1 antibody [3G10] (ab115548) + Human pancreas tissue lysate

    Predicted band size : 83 kDa
  • ab115548, at 10 µg/ml, staining DAZ1 in HeLa cell by Immunofluorescence.
  • Detection limit for ab115548 is approximately 0.3 ng/ml as a capture antibody.

References for Anti-DAZ1 antibody [3G10] (ab115548)

ab115548 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"