Overview

  • Product name
  • Description
    Rabbit polyclonal to DAZ3
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-201 of Human DAZ3 (Uniprot ID: Q9NR90).

  • Positive control
    • HeLa whole cell lysate; HeLa cells; Human colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 54 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    RNA-binding protein that plays an essential role in spermatogenesis. May act by binding to the 3'-UTR of mRNAs and regulating their translation.
  • Tissue specificity
    Testis specific.
  • Involvement in disease
    Spermatogenic failure Y-linked 2
  • Sequence similarities
    Belongs to the RRM DAZ family.
    Contains 12 DAZ-like domains.
    Contains 1 RRM (RNA recognition motif) domain.
  • Domain
    The DAZ-like repeats are essential and mediate the interaction with DAZAP1 and DAZAP2.
  • Cellular localization
    Cytoplasm. Nucleus. Predominantly cytoplasmic. Nuclear at some stages of spermatozoide development. Localizes both to the nuclei and cytoplasm of spermatozoide differentiation. Nuclear in fetal gonocytes and in spermatogonial nuclei. It then relocates to the cytoplasm during male meiosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • DAZ3 antibody
    • DAZ3_HUMAN antibody
    • deleted in azoospermia 3 antibody
    • Deleted in azoospermia protein 3 antibody
    • MGC126441 antibody
    • pDP1679 antibody
    see all

Images

  • Anti-DAZ3 antibody (ab154915) at 1/500 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size : 54 kDa
  • Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling DAZ3 with ab154915 at 1/250 dilution.
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling DAZ3 with ab154915 at 1/500 dilution (green). Lower image shows cells co-stained with Hoechst 33342.

References

ab154915 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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