The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionRNA-binding protein that plays an essential role in spermatogenesis. May act by binding to the 3'-UTR of mRNAs and regulating their translation.
Tissue specificityTestis specific.
Involvement in diseaseSpermatogenic failure Y-linked 2
Sequence similaritiesBelongs to the RRM DAZ family. Contains 12 DAZ-like domains. Contains 1 RRM (RNA recognition motif) domain.
DomainThe DAZ-like repeats are essential and mediate the interaction with DAZAP1 and DAZAP2.
Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic. Nuclear at some stages of spermatozoide development. Localizes both to the nuclei and cytoplasm of spermatozoide differentiation. Nuclear in fetal gonocytes and in spermatogonial nuclei. It then relocates to the cytoplasm during male meiosis.