Overview

  • Product nameAnti-DAZAP1 antibody [2242C2a]
    See all DAZAP1 primary antibodies
  • Description
    Mouse monoclonal [2242C2a] to DAZAP1
  • Tested applicationsSuitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment comprising of 50-200 amino acids from near the C terminus of Human DAZAP1 (NM_170711).

  • Positive control
    • Recombinant fragment of human DAZAP1.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS,(8.0mM Sodium phosphate, 3.0mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate), pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab76578 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
  • ClonalityMonoclonal
  • Clone number2242C2a
  • IsotypeIgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 2 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 41 kDa).
Dot blot Use at an assay dependent dilution.

Target

  • FunctionRNA-binding protein, which may be required during spermatogenesis.
  • Tissue specificityMainly expressed in testis. Expressed to a lower level in thymus. Weakly or not expressed in heart, liver, brain, placenta, lung, skeletal muscle, kidney and pancreas.
  • Sequence similaritiesContains 2 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Nuclear at some stages of spermatozoides development. In midpachytene spermatocytes, it is localized in both the cytoplasm and the nuclei and is clearly excluded from the sex vesicles. In round spermatids, it localizes mainly in the nuclei, whereas in elongated spermatids, it localizes to the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • DAZ associated protein 1 antibody
    • DAZ-associated protein 1 antibody
    • Dazap1 antibody
    • DAZP1_HUMAN antibody
    • Deleted in azoospermia associated protein 1 antibody
    • Deleted in azoospermia-associated protein 1 antibody
    see all

Anti-DAZAP1 antibody [2242C2a] images

References for Anti-DAZAP1 antibody [2242C2a] (ab76578)

ab76578 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"