Recombinant
RabMAb

Anti-DAZL antibody [EPR5140] (ab108372)

Overview

  • Product name
    Anti-DAZL antibody [EPR5140]
    See all DAZL primary antibodies
  • Description
    Rabbit monoclonal [EPR5140] to DAZL
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    A synthetic peptide corresponding to residues in Human DAZL.

  • Positive control
    • Human testis and Human small intestine lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab108372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function
      RNA-binding protein, which is essential for gametogenesis. Plays a central role during spermatogenesis. May act by binding to the 3'-UTR of mRNA and thereby regulating the translation of key transcripts.
    • Tissue specificity
      Testis specific.
    • Sequence similarities
      Belongs to the RRM DAZ family.
      Contains 1 DAZ-like domain.
      Contains 1 RRM (RNA recognition motif) domain.
    • Domain
      The DAZ-like domain mediates the interaction with DAZAP1 and DAZAP2.
    • Cellular localization
      Cytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Nuclear in spermatogonia until near the end of the meiotic prophase and cytoplasmic localization from then onward.
    • Information by UniProt
    • Database links
    • Alternative names
      • DAZ homolog antibody
      • DAZ like autosomal antibody
      • DAZ-like autosomal antibody
      • DAZH antibody
      • DAZL 1 antibody
      • DAZL antibody
      • DAZL_HUMAN antibody
      • DAZL1 antibody
      • DAZLA antibody
      • Deleted in azoospermia like 1 antibody
      • Deleted in azoospermia like antibody
      • Deleted in azoospermia like autosomal antibody
      • Deleted in azoospermia-like 1 antibody
      • Deleted in azoospermia-like antibody
      • Germline specific RNA binding protein antibody
      • MGC26406 antibody
      • Spermatogenesis gene on the Y like autosomal antibody
      • SPGY like autosomal antibody
      • SPGY-like-autosomal antibody
      • SPGYLA antibody
      • Tpx2 antibody
      see all

    Images

    • All lanes : Anti-DAZL antibody [EPR5140] (ab108372) at 1/1000 dilution

      Lane 1 : Human testis lysate
      Lane 2 : Human small intestine lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 33 kDa

    References

    ab108372 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Testis, adult)
    Permeabilization
    Yes - 0.1% Triton X-100 in PBS
    Specification
    Testis, adult
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Username

    Mr. Bryan Niedenberger

    Verified customer

    Submitted Mar 11 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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