Anti-DAZL antibody [EPR5140] (ab108372)

Overview

  • Product nameAnti-DAZL antibody [EPR5140]
    See all DAZL primary antibodies
  • Description
    Rabbit monoclonal [EPR5140] to DAZL
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    A synthetic peptide corresponding to residues in Human DAZL.

  • Positive control
    • Human testis and Human small intestine lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab108372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
  • Application notesIs unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • FunctionRNA-binding protein, which is essential for gametogenesis. Plays a central role during spermatogenesis. May act by binding to the 3'-UTR of mRNA and thereby regulating the translation of key transcripts.
    • Tissue specificityTestis specific.
    • Sequence similaritiesBelongs to the RRM DAZ family.
      Contains 1 DAZ-like domain.
      Contains 1 RRM (RNA recognition motif) domain.
    • DomainThe DAZ-like domain mediates the interaction with DAZAP1 and DAZAP2.
    • Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Nuclear in spermatogonia until near the end of the meiotic prophase and cytoplasmic localization from then onward.
    • Information by UniProt
    • Database links
    • Alternative names
      • DAZ homolog antibody
      • DAZ like autosomal antibody
      • DAZ-like autosomal antibody
      • DAZH antibody
      • DAZL 1 antibody
      • DAZL antibody
      • DAZL_HUMAN antibody
      • DAZL1 antibody
      • DAZLA antibody
      • Deleted in azoospermia like 1 antibody
      • Deleted in azoospermia like antibody
      • Deleted in azoospermia like autosomal antibody
      • Deleted in azoospermia-like 1 antibody
      • Deleted in azoospermia-like antibody
      • Germline specific RNA binding protein antibody
      • MGC26406 antibody
      • Spermatogenesis gene on the Y like autosomal antibody
      • SPGY like autosomal antibody
      • SPGY-like-autosomal antibody
      • SPGYLA antibody
      • Tpx2 antibody
      see all

    Anti-DAZL antibody [EPR5140] images

    • All lanes : Anti-DAZL antibody [EPR5140] (ab108372) at 1/1000 dilution

      Lane 1 : Human testis lysate
      Lane 2 : Human small intestine lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 33 kDa

    References for Anti-DAZL antibody [EPR5140] (ab108372)

    ab108372 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Testis, adult)
    Permeabilization Yes - 0.1% Triton X-100 in PBS
    Specification Testis, adult
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative Paraformaldehyde
    Username

    Mr. Bryan Niedenberger

    Verified customer

    Submitted Mar 11 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"