Overview

  • Product name
  • Description
    Goat polyclonal to DBF4
  • Specificity
    ab116613 is expected to recognize both reported isoforms (NP_038754.1; NP_001177646.1).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    DKKRTEYLPAHEDRT

    with a Cysteine residue linker, corresponding to internal sequence amino acids 585-599 of Mouse DBF4 (NP_038754.1; NP_001177646.1).

  • Positive control
    • Mouse and Rat Testis lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab116613 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab116613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 74 kDa.

Target

  • Function
    Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
  • Tissue specificity
    Highly expressed in testis and thymus. Expressed also in most cancer cells lines.
  • Sequence similarities
    Contains 2 BRCT domains.
    Contains 1 DBF4-type zinc finger.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activator of S phase kinase antibody
    • ASK antibody
    • CHIF antibody
    • Chiffon homolog A antibody
    • DBF4 antibody
    • DBF4 homolog (S. cerevisiae) antibody
    • DBF4 homolog antibody
    • DBF4 type zinc finger containing protein 1 antibody
    • DBF4 zinc finger A antibody
    • DBF4 zinc finger antibody
    • DBF4, S. cerevisiae, homolog of antibody
    • DBF4-type zinc finger-containing protein 1 antibody
    • DBF4A antibody
    • DBF4A_HUMAN antibody
    • Dumbbell former 4, S. cerevisiae, homolog of antibody
    • OTTHUMP00000161661 antibody
    • Protein DBF4 homolog A antibody
    • ZDBF1 antibody
    • Zinc finger, DBF-type containing 1 antibody
    see all

Images

  • Anti-DBF4 antibody (ab116613) at 1 µg/ml + Mouse testis lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 74 kDa

References

ab116613 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab116613.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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