Overview

  • Product nameAnti-DBF4 antibody
    See all DBF4 primary antibodies
  • Description
    Mouse monoclonal to DBF4
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: NSGAMRIHSK GHFQGGIQVK NEKNRPSLKS LKTDNRPEKS KCKPLWGKVF YLDLPSVTIS EKLQKDIKDL GGRVEEFLSK DISYLISNKK EAKFAQT, corresponding to amino acids 2-99 of Human DBF4

Properties

Applications

Our Abpromise guarantee covers the use of ab56813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionRegulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
    • Tissue specificityHighly expressed in testis and thymus. Expressed also in most cancer cells lines.
    • Sequence similaritiesContains 2 BRCT domains.
      Contains 1 DBF4-type zinc finger.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activator of S phase kinase antibody
      • ASK antibody
      • CHIF antibody
      • Chiffon homolog A antibody
      • DBF4 antibody
      • DBF4 homolog (S. cerevisiae) antibody
      • DBF4 homolog antibody
      • DBF4 type zinc finger containing protein 1 antibody
      • DBF4 zinc finger A antibody
      • DBF4 zinc finger antibody
      • DBF4, S. cerevisiae, homolog of antibody
      • DBF4-type zinc finger-containing protein 1 antibody
      • DBF4A antibody
      • DBF4A_HUMAN antibody
      • Dumbbell former 4, S. cerevisiae, homolog of antibody
      • OTTHUMP00000161661 antibody
      • Protein DBF4 homolog A antibody
      • ZDBF1 antibody
      • Zinc finger, DBF-type containing 1 antibody
      see all

    Anti-DBF4 antibody images

    • BDF4 antibody (ab56813) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human spleen.
    • Western blot against tagged recombinant protein immunogen using ab56813 BDF4 antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa

    References for Anti-DBF4 antibody (ab56813)

    ab56813 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab56813.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"