• Product name
  • Description
    Rabbit polyclonal to DBF4
  • Specificity
    ab70134 detects endogenous levels of total DBF4 protein.
  • Tested applications
    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from the N terminus of human DBF4.

  • Positive control
    • NIH3T3 cell extracts treated with H2O2 (100µM, 30mins), COS7 cell extracts treated with PMA (125mg/ml, 30mins), and NIH3T3 cell extracts treated with both H2O2 (100µM, 30mins) and PMA (125mg/ml, 30mins).


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab70124 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab70134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
ELISA 1/10000.


  • Function
    Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
  • Tissue specificity
    Highly expressed in testis and thymus. Expressed also in most cancer cells lines.
  • Sequence similarities
    Contains 2 BRCT domains.
    Contains 1 DBF4-type zinc finger.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Activator of S phase kinase antibody
    • ASK antibody
    • CHIF antibody
    • Chiffon homolog A antibody
    • DBF4 antibody
    • DBF4 homolog (S. cerevisiae) antibody
    • DBF4 homolog antibody
    • DBF4 type zinc finger containing protein 1 antibody
    • DBF4 zinc finger A antibody
    • DBF4 zinc finger antibody
    • DBF4, S. cerevisiae, homolog of antibody
    • DBF4-type zinc finger-containing protein 1 antibody
    • DBF4A antibody
    • DBF4A_HUMAN antibody
    • Dumbbell former 4, S. cerevisiae, homolog of antibody
    • OTTHUMP00000161661 antibody
    • Protein DBF4 homolog A antibody
    • ZDBF1 antibody
    • Zinc finger, DBF-type containing 1 antibody
    see all


  • All lanes : Anti-DBF4 antibody (ab70134) at 1/500 dilution

    Lane 1 : NIH3T3 cell extracts treated with H2O2 (100µM, 30mins) at 5 µg
    Lane 2 : COS7 cell extracts treated with PMA (125mg/ml, 30mins)
    Lane 3 : NIH3T3 cell extracts treated with H2O2 (100µM, 30mins) and PMA (125mg/ml, 30mins) with immunising peptide at 5 µg

    Predicted band size : 77 kDa
    Observed band size : 77 kDa
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab70134 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70134, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


ab70134 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us in regards to Anti-DBF4 antibody (ab70134). I have been assured by the source of this antibody that the Western blot performed and displayed on the datasheet was performed with endogenous expression of DBF4 from NIH3T3 a...

Read More


Sign up