Anti-DBF4 antibody [EPR5635] (ab124707)

Overview

  • Product nameAnti-DBF4 antibody [EPR5635]
    See all DBF4 primary antibodies
  • Description
    Rabbit monoclonal [EPR5635] to DBF4
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human DBF4 protein (Q9UBU7).

  • Positive control
    • PC-3, Jurkat, SW480, and HepG2 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab124707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 77 kDa.
  • Application notesIs unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • FunctionRegulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
    • Tissue specificityHighly expressed in testis and thymus. Expressed also in most cancer cells lines.
    • Sequence similaritiesContains 2 BRCT domains.
      Contains 1 DBF4-type zinc finger.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activator of S phase kinase antibody
      • ASK antibody
      • CHIF antibody
      • Chiffon homolog A antibody
      • DBF4 antibody
      • DBF4 homolog (S. cerevisiae) antibody
      • DBF4 homolog antibody
      • DBF4 type zinc finger containing protein 1 antibody
      • DBF4 zinc finger A antibody
      • DBF4 zinc finger antibody
      • DBF4, S. cerevisiae, homolog of antibody
      • DBF4-type zinc finger-containing protein 1 antibody
      • DBF4A antibody
      • DBF4A_HUMAN antibody
      • Dumbbell former 4, S. cerevisiae, homolog of antibody
      • OTTHUMP00000161661 antibody
      • Protein DBF4 homolog A antibody
      • ZDBF1 antibody
      • Zinc finger, DBF-type containing 1 antibody
      see all

    Anti-DBF4 antibody [EPR5635] images

    • All lanes : Anti-DBF4 antibody [EPR5635] (ab124707) at 1/10000 dilution

      Lane 1 : PC-3 cell lysates
      Lane 2 : Jurkat cell lysates
      Lane 3 : SW480 cell lysates
      Lane 4 : HepG2 cell lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 77 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-DBF4 antibody [EPR5635] (ab124707)

    ab124707 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunoprecipitation
    Sample Human Cell lysate - whole cell (hEK293)
    Total protein in input 300 µg
    Immuno-precipitation step Other - protein G dynabeads
    Specification hEK293
    Username

    Dr. Edyta Marcon

    Verified customer

    Submitted Jun 27 2015

    Application Western blot
    Loading amount 10 µg
    Gel Running Conditions Reduced Denaturing (4-12 Bis tris)
    Sample Human Cell lysate - whole cell (293T)
    Specification 293T
    Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 14 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HEK293T)
    Loading amount 50 µg
    Specification HEK293T
    Gel Running Conditions Reduced Denaturing (8% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 27 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"