Overview

  • Product nameAnti-DBF4B antibody
    See all DBF4B primary antibodies
  • Description
    Mouse polyclonal to DBF4B
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human DBF4B protein (NP_079380.1)

  • Positive control
    • DBF4B transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab69154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/500 - 1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: Isoform 4, 18 kDa; Isoform 1, 67 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionRegulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases. The complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle.
    • Tissue specificityWidely expressed. Highly expressed in testis.
    • Sequence similaritiesContains 1 BRCT domain.
      Contains 1 DBF4-type zinc finger.
    • Developmental stageIncreases as cells enter in S phase through G2/M phase. The protein has a short half-life (at protein level).
    • Post-translational
      modifications
      Phosphorylated.
    • Cellular localizationNucleus. Predominantly found in soluble fraction but not in the chromatin-bound fraction.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activator of S phase kinase like protein 1 antibody
      • Activator of S phase kinase-like protein 1 antibody
      • ASK like protein 1 antibody
      • ASK-like protein 1 antibody
      • ASKL1 antibody
      • chifb antibody
      • Chiffon homolog B antibody
      • DBF 4B antibody
      • DBF4 homolog B (S. cerevisiae) antibody
      • DBF4 homolog B antibody
      • Dbf4 related factor 1 antibody
      • Dbf4-related factor 1 antibody
      • dbf4b antibody
      • DBF4B_HUMAN antibody
      • DRF1 antibody
      • FLJ13087 antibody
      • MGC15009 antibody
      • Protein DBF4 homolog B antibody
      • ZDBF1B antibody
      • Zinc finger DBF type containing 1B antibody
      see all

    Anti-DBF4B antibody images

    • All lanes : Anti-DBF4B antibody (ab69154) at 1/500 dilution

      Lane 1 : DBF4B transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 18 kDa
      Observed band size : 18 kDa

    References for Anti-DBF4B antibody (ab69154)

    ab69154 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"